Piórkowska Katarzyna, Żukowski Kacper, Ropka-Molik Katarzyna, Tyra Mirosław, Gurgul Artur
Department of Animal Molecular Biology, National Research Institute of Animal Production, Balice, Poland.
Department of Cattle Breeding, National Research Institute of Animal Production, Balice, Poland.
Genet Mol Biol. 2018 Jan-Mar;41(1):125-136. doi: 10.1590/1678-4685-GMB-2016-0101.
Pork is the most popular meat in the world. Unfortunately, the selection pressure focused on high meat content led to a reduction in pork quality. The present study used RNA-seq technology to identify metabolic process genes related to pork quality traits and fat deposition. Differentially expressed genes (DEGs) were identified between pigs of Pulawska and Polish Landrace breeds for two the most important muscles (semimembranosus and longissimus dorsi). A total of 71 significant DEGs were reported: 15 for longissimus dorsi and 56 for semimembranosus muscles. The genes overexpressed in Pulawska pigs were involved in lipid metabolism (APOD, LXRA, LIPE, AP2B1, ENSSSCG00000028753 and OAS2) and proteolysis (CST6, CTSD, ISG15 and UCHL1). In Polish Landrace pigs, genes playing a role in biological adhesion (KIT, VCAN, HES1, SFRP2, CDH11, SSX2IP and PCDH17), actin cytoskeletal organisation (FRMD6, LIMK1, KIF23 and CNN1) and calcium ion binding (PVALB, CIB2, PCDH17, VCAN and CDH11) were transcriptionally more active. The present study allows for better understanding of the physiological processes associated with lipid metabolism and muscle fiber organization. This information could be helpful in further research aiming to estimate the genetic markers.
猪肉是世界上最受欢迎的肉类。不幸的是，专注于高肉含量的选择压力导致猪肉品质下降。本研究使用RNA测序技术来鉴定与猪肉品质性状和脂肪沉积相关的代谢过程基因。在普瓦夫斯卡猪和波兰大白猪的两种最重要肌肉（半膜肌和背最长肌）之间鉴定出差异表达基因（DEG）。共报告了71个显著的DEG：背最长肌中有15个，半膜肌中有56个。在普瓦夫斯卡猪中过表达的基因参与脂质代谢（载脂蛋白D、肝脏X受体α、激素敏感性脂肪酶、衔接蛋白2β1、ENS SSCG00000028753和2'-5'-寡腺苷酸合成酶2）和蛋白水解（胱抑素F、组织蛋白酶D、干扰素刺激基因15和泛素羧基末端水解酶L1）。在波兰大白猪中，参与生物黏附（原癌基因c-Kit、多功能蛋白聚糖、毛状分裂增强子1、分泌型卷曲相关蛋白2、钙黏蛋白11、滑膜肉瘤X染色体结合蛋白2和原钙黏蛋白17）、肌动蛋白细胞骨架组织（FERM结构域蛋白6、丝裂原活化蛋白激酶相互作用蛋白1、驱动蛋白家族成员23和肌动蛋白结合蛋白1）和钙离子结合（小白蛋白、钙结合蛋白2、原钙黏蛋白17、多功能蛋白聚糖和钙黏蛋白11）的基因转录活性更高。本研究有助于更好地理解与脂质代谢和肌纤维组织相关的生理过程。这些信息可能有助于进一步研究以估计遗传标记。
