Yuan Yuan, Zhang Peijun, Wang Kun, Liu Mingzhong, Li Jing, Zheng Jingsong, Wang Ding, Xu Wenjie, Lin Mingli, Dong Lijun, Zhu Chenglong, Qiu Qiang, Li Songhai
Center for Ecological and Environmental Sciences, Northwestern Polytechnical University, Xi'an 710072, China.
Qingdao Research Institute, Northwestern Polytechnical University, Qingdao 266200, China.
Genes (Basel). 2018 Apr 16;9(4):213. doi: 10.3390/genes9040213.
The Yangtze finless porpoise ( ssp. ) is a subspecies of the narrow-ridged finless porpoise (). In total, 714.28 gigabases (Gb) of raw reads were generated by whole-genome sequencing of the Yangtze finless porpoise, using an Illumina HiSeq 2000 platform. After filtering the low-quality and duplicated reads, we assembled a draft genome of 2.22 Gb, with contig N50 and scaffold N50 values of 46.69 kilobases (kb) and 1.71 megabases (Mb), respectively. We identified 887.63 Mb of repetitive sequences and predicted 18,479 protein-coding genes in the assembled genome. The phylogenetic tree showed a relationship between the Yangtze finless porpoise and the Yangtze River dolphin, which diverged approximately 20.84 million years ago. In comparisons with the genomes of 10 other mammals, we detected 44 species-specific gene families, 164 expanded gene families, and 313 positively selected genes in the Yangtze finless porpoise genome. The assembled genome sequence and underlying sequence data are available at the National Center for Biotechnology Information under BioProject accession number PRJNA433603.
长江江豚是窄脊江豚的一个亚种。利用Illumina HiSeq 2000平台对长江江豚进行全基因组测序，共产生了714.28千兆碱基（Gb）的原始读数。在过滤掉低质量和重复的读数后，我们组装了一个2.22 Gb的基因组草图，重叠群N50和支架N50值分别为46.69千碱基（kb）和1.71兆碱基（Mb）。我们在组装的基因组中鉴定出887.63 Mb的重复序列，并预测了18,479个蛋白质编码基因。系统发育树显示了长江江豚与长江江豚之间的关系，它们大约在2084万年前分化。在与其他10种哺乳动物的基因组比较中，我们在长江江豚基因组中检测到44个物种特异性基因家族、164个扩增基因家族和313个正选择基因。组装的基因组序列和基础序列数据可在国家生物技术信息中心获得，生物项目登录号为PRJNA433603。
