Department of Mechanical Engineering, National Kaohsiung University of Science and Technology, No. 415, Jiangong Rd., Sanmin Dist., Kaohsiung City 807, Taiwan.
Department of Aeronautical and Mechanical Engineering, Air Force Academy, Academy, No. Sisou 1, Jieshou W. Road, Kaohsiung 820, Taiwan.
Sensors (Basel). 2018 Apr 16;18(4):1217. doi: 10.3390/s18041217.
In this study, we proposed a U-shaped optical fiber probe fabricated using a flame heating method. The probe was packaged in glass tube to reduce human factors during experimental testing of the probe as a glucose sensor. The U-shaped fiber probe was found to have high sensitivity in detecting the very small molecule. When the sensor was dipped in solutions with different refractive indexes, its wavelength or transmission loss changed. We used electrostatic self-assembly to bond gold nanoparticles and glucose oxidase (GOD) onto the sensor’s surface. The results over five cycles of the experiment showed that, as the glucose concentration increased, the refractive index of the sensor decreased and its spectrum wavelength shifted. The best wavelength sensitivity was 2.899 nm/%, and the linearity was 0.9771. The best transmission loss sensitivity was 5.101 dB/%, and the linearity was 0.9734. Therefore, the proposed U-shaped optical fiber probe with gold nanoparticles and GOD has good potential for use as a blood sugar sensor in the future.
在这项研究中，我们提出了一种使用火焰加热法制造的 U 型光纤探头。该探头被封装在玻璃管中，以减少探头作为葡萄糖传感器在实验测试过程中人为因素的影响。研究发现，U 型光纤探头在检测非常小的分子时具有很高的灵敏度。当传感器浸入具有不同折射率的溶液中时，其波长或传输损耗会发生变化。我们使用静电自组装将金纳米粒子和葡萄糖氧化酶（GOD）键合到传感器表面。经过五次实验循环的结果表明，随着葡萄糖浓度的增加，传感器的折射率降低，其光谱波长发生移动。最佳波长灵敏度为 2.899nm/%，线性度为 0.9771。最佳传输损耗灵敏度为 5.101dB/%，线性度为 0.9734。因此，具有金纳米粒子和 GOD 的这种新型 U 型光纤探头在未来有作为血糖传感器的良好应用潜力。
