一种用于 microRNA 的基于环形光纤的微腔生物传感器探测器。
A Lamping U-Shaped Fiber Biosensor Detector for MicroRNA.
机构信息
Department of Mechanical Engineering, National Kaohsiung University of Science and Technology, 415 Chien Kung Road, Kaohsiung 80778, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Fooyin University, Kaohsiung 83102, Taiwan.
出版信息
Sensors (Basel). 2020 Mar 9;20(5):1509. doi: 10.3390/s20051509.
This study presents a U-shaped optical fiber developed for a facile application of microRNA detection. It is fabricated by the lamping process and packaged in a quartz tube to eliminate human negligence. In addition, silanization and electrostatic self-assembly are employed to bind gold nanoparticles and miRNA-133a probe onto the silicon dioxide of the fiber surface. For Mahlavu of hepatocellular carcinoma (HCC), detection is determined by the wavelength shift and transmission loss of a U-shaped optical fiber biosensor. The spectral sensitivity of wavelength and their coefficient of determination are found at -218.319 nm/ ng/mL and 0.839, respectively. Concurrently, the sensitivity of transmission loss and their coefficient of determination are found at 162.394 dB/ ng/mL and 0.984, respectively. A method for estimating the limit of detection of Mahlavu is at 0.0133 ng/mL. The results show that the proposed U-shaped biosensor is highly specific to miRNA-133a and possesses good sensitivity to variations in specimen concentration. As such, it could be of substantial value in microRNA detection.
本研究提出了一种 U 型光纤，用于简便地进行 microRNA 检测。它是通过拉制工艺制造的，并封装在石英管中，以消除人为疏忽。此外，还采用硅烷化和静电自组装将金纳米粒子和 miRNA-133a 探针结合到光纤表面的二氧化硅上。对于肝癌（HCC）的 Mahlavu，通过 U 型光纤生物传感器的波长位移和传输损耗来确定检测。发现波长的光谱灵敏度及其决定系数分别为-218.319nm/ng/mL 和 0.839。同时，传输损耗的灵敏度及其决定系数分别为 162.394dB/ng/mL 和 0.984。估计 Mahlavu 检测限的方法为 0.0133ng/mL。结果表明，所提出的 U 型生物传感器对 miRNA-133a 具有高度特异性，并且对标本浓度变化具有良好的灵敏度。因此，它在 microRNA 检测中具有重要价值。
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