利用 ANCHOR DNA 标记系统实时成像真核细胞中的特定基因组位点。
Real-time imaging of specific genomic loci in eukaryotic cells using the ANCHOR DNA labelling system.
机构信息
Laboratoire de Biologie Moléculaire Eucaryote (LBME), Centre de Biologie Intégrative (CBI), University of Toulouse, CNRS, 118 route de Narbonne, 31062 Toulouse, France.
Laboratoire de Microbiologie et Génétique Moléculaires (LMGM), Centre de Biologie Intégrative (CBI), University of Toulouse, CNRS, 118 route de Narbonne, 31062 Toulouse, France.
出版信息
Methods. 2018 Jun 1;142:16-23. doi: 10.1016/j.ymeth.2018.04.008. Epub 2018 Apr 13.
Spatio-temporal organization of the cell nucleus adapts to and regulates genomic processes. Microscopy approaches that enable direct monitoring of specific chromatin sites in single cells and in real time are needed to better understand the dynamics involved. In this chapter, we describe the principle and development of ANCHOR, a novel tool for DNA labelling in eukaryotic cells. Protocols for use of ANCHOR to visualize a single genomic locus in eukaryotic cells are presented. We describe an approach for live cell imaging of a DNA locus during the entire cell cycle in human breast cancer cells.
细胞核的时空组织适应并调节基因组过程。需要能够直接监测单细胞和实时特定染色质位点的显微镜方法，以便更好地理解所涉及的动态。在本章中，我们描述了用于真核细胞中 DNA 标记的新型工具 ANCHOR 的原理和开发。介绍了用于在真核细胞中可视化单个基因组基因座的 ANCHOR 的使用方案。我们描述了一种在人类乳腺癌细胞整个细胞周期中对 DNA 基因座进行活细胞成像的方法。
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