Generation of an iPS cell line via a non-integrative method using urine-derived cells from a patient with USH2A-associated retinitis pigmentosa.

作者信息

Guo Yonglong, Zeng Qiaolang, Liu Shiwei, Yu Quan, Wang Peiyuan, Ma Hongjie, Shi Shanshan, Yan Xin, Cui Zekai, Xie Mengyuan, Xue Yunxia, Zha Qingbing, Li Zhijie, Zhang Jun, Tang Shibo, Chen Jiansu

机构信息

Key Laboratory for Regenerative Medicine, Ministry of Education, Jinan University, Guangzhou 510632, China.

The Department of Ophthalmology, The First Affiliated Hospital, Jinan University, Guangzhou 510632, China.

出版信息

Stem Cell Res. 2018 May;29:139-142. doi: 10.1016/j.scr.2018.03.022. Epub 2018 Apr 6.

We have established an induced pluripotent stem (iPS) cell line using urine-derived cells from a 27-year-old male patient with retinitis pigmentosa associated with point mutations in the USH2A gene. Feeder-free culture conditions and the integration-free CytoTune™-iPS 2.0 Sendai Reprogramming Kit were used.