非小细胞肺癌患者血浆外泌体水平：与临床病理特征的相关性及其预后意义。

Plasma exosome levels in non-small-cell lung cancer: Correlation with clinicopathological features and prognostic implications.

机构信息

Department of Epidemiology, College of Preventive Medicine, Army Medical University (Third Military Medical University), Chongqing 400038, China.

Department of Thoracic Surgery, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing 400038, China.

出版信息

Cancer Biomark. 2018;22(2):267-274. doi: 10.3233/CBM-170955.

DOI:10.3233/CBM-170955

PMID:29660899

Abstract

BACKGROUND

Biomarker studies revealed important clinical significance of exosome for cancer patients. However, there is currently no consensus on exosome quantification methods.

METHODS

Bicinchoninic acid (BCA) method, acetylcholinesterase (AChE) method and nanoparticle tracking analysis (NTA) were utilized to quantify 20 plasma exosome samples, and interrelations between these three methods were explored. Associations of plasma exosome levels with characteristics and prognosis of 208 non-small-cell lung cancer (NSCLC) patients were investigated.

RESULTS

Results of the three methods for exosome quantification were significantly correlated with each other. Correlation coefficient between AChE and NTA (r= 0.79, P< 0.001) was greater than that between BCA and NTA (r= 0.64, P= 0.003). Plasma exosome levels of 208 NSCLC patients were then quantified with AChE method. Exosome level was significantly associated with tumour stage (P< 0.001) and the history of chronic obstructive pulmonary disease (P= 0.023). Cox proportional hazard analysis demonstrated that higher exosome level was independently associated with poorer overall survival (P= 0.033; hazard ratio = 1.72, 95% confidence interval: 1.05-2.83).

CONCLUSIONS

Plasma exosome level correlates with tumor stage and the history of chronic obstructive pulmonary disease, and may serve as a prognostic factor for NSCLC.

摘要

背景

生物标志物研究显示外泌体对癌症患者具有重要的临床意义。然而，目前对于外泌体的定量方法尚无共识。

方法

使用双缩脲法（BCA）、乙酰胆碱酯酶（AChE）法和纳米颗粒跟踪分析（NTA）对 20 个血浆外泌体样本进行定量，并探讨了这三种方法之间的相互关系。研究了血浆外泌体水平与 208 例非小细胞肺癌（NSCLC）患者特征和预后的相关性。

结果

三种外泌体定量方法的结果相互显著相关。AChE 与 NTA 之间的相关系数（r=0.79，P<0.001）大于 BCA 与 NTA 之间的相关系数（r=0.64，P=0.003）。然后使用 AChE 法对 208 例 NSCLC 患者的血浆外泌体水平进行了定量。外泌体水平与肿瘤分期（P<0.001）和慢性阻塞性肺疾病史（P=0.023）显著相关。Cox 比例风险分析表明，较高的外泌体水平与总生存期较差独立相关（P=0.033；风险比=1.72，95%置信区间：1.05-2.83）。