Nanomaterials Research Institute (NMRI), National Institute of Advanced Industrial Science and Technology (AIST), Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Nanoscale. 2018 May 17;10(19):8969-8978. doi: 10.1039/c8nr00641e.
A nanodiamond (ND) is a promising material for drug delivery applications owing to its relatively low cost, amenability to large-scale synthesis, unique structure, and low toxicity. However, synthesizing drug-loaded ND conjugates with uniform and tunable sizes, high loading capacity, efficacy in drug delivery, and versatility in terms of surface functionalization has been challenging. Here, we show that perfluorooctanoic acid-functionalized NDs spontaneously transform into well-dispersed and biocompatible supraparticle (SP) nanoclusters. We demonstrate that the synthesized ND-based SPs (ND-SPs) exhibit high penetration through the cell membrane and are therefore superior as drug carriers for conventional nanomedicines such as polyethylene glycol and phospholipid-based nanocapsules and simple drug-loaded ND conjugates. We confirm the efficacy of ND-SPs in the eradication of cancer cells in vitro and in vivo. Our results demonstrate that the synthesized ND-SPs are useful for targeted drug delivery in a variety of biological applications.
纳米金刚石（ND）是一种很有前途的药物输送应用材料，因为它的成本相对较低、适合大规模合成、具有独特的结构和低毒性。然而，合成具有均匀和可调尺寸、高载药量、高效药物输送和多功能表面功能化的载药 ND 缀合物一直具有挑战性。在这里，我们表明，全氟辛酸官能化的 ND 会自发转化为分散良好且生物相容的超粒子（SP）纳米簇。我们证明，合成的基于 ND 的 SP（ND-SP）表现出高穿透细胞膜的能力，因此作为药物载体优于传统的纳米药物，如聚乙二醇和基于磷脂的纳米胶囊以及简单的载药 ND 缀合物。我们证实了 ND-SP 在体外和体内消除癌细胞的功效。我们的结果表明，合成的 ND-SP 可用于各种生物应用中的靶向药物输送。
