Division of Nanobiotechnology, Faculty of Animal Sciences, Warsaw University of Life Sciences, Ciszewskiego 8, 02-786 Warsaw, Poland.
Department of Neurosurgery, Oncology Center- Maria Sklodowska Curie Memorial, Warsaw, Roentgena 5, 02-781 Warsaw, Poland.
Molecules. 2019 Apr 19;24(8):1549. doi: 10.3390/molecules24081549.
Our previous studies have shown that diamond nanoparticles (NDs) exhibited antiangiogenic and proapoptotic properties in vitro in glioblastoma multiforme (GBM) cells and in tumors in vivo. Moreover, NDs inhibited adhesion, leading to the suppression of migration and invasion of GBM. In the present study, we hypothesized that the NDs might also inhibit proliferation and cell cycle in glioma cells. Experiments were performed in vitro with the U87 and U118 lines of GBM cells, and for comparison, the Hs5 line of stromal cells (normal cells) after 24 h and 72 h of treatment. The analyses included cell morphology, cell death, viability, and cell cycle analysis, double timing assay, and gene expression (). After 72 h of ND treatment, the expression level of , and in the U118 cells, and , , and in the U87 cells were significantly lower in comparison to those in the control group. We observed that decreased expression of cyclins inhibited the G1/S phase transition, arresting the cell cycle in the G0/G1 phase in glioma cells. The NDs did not affect the cell cycle as well as and expression in normal cells (Hs5), although it can be assumed that the NDs reduced proliferation and altered the cell cycle in fast dividing cells.
我们之前的研究表明，金刚石纳米粒子（NDs）在体外的多形性胶质母细胞瘤（GBM）细胞和体内肿瘤中表现出抗血管生成和促凋亡的特性。此外，NDs 抑制了细胞的黏附，从而抑制了 GBM 的迁移和侵袭。在本研究中，我们假设 NDs 也可能抑制神经胶质瘤细胞的增殖和细胞周期。在体外进行了 U87 和 U118 两种 GBM 细胞系的实验，并进行了比较，对间质细胞（正常细胞）Hs5 系进行了 24 h 和 72 h 处理后的实验。分析包括细胞形态、细胞死亡、活力和细胞周期分析、双时间测定和基因表达（）。在 NDs 处理 72 h 后，U118 细胞中的 和 ，以及 U87 细胞中的 、 、 和 表达水平明显低于对照组。我们观察到，细胞周期蛋白表达的下调抑制了 G1/S 期转变，使神经胶质瘤细胞的细胞周期停滞在 G0/G1 期。NDs 对正常细胞（Hs5）的细胞周期以及 和 表达没有影响，尽管可以假设 NDs 减少了增殖并改变了快速分裂细胞的细胞周期。
