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筛选和鉴定园艺土壤真菌,以评估它们对植物寄生线虫 Nacobbus aberrans 的防治效果。

Screening and identification of horticultural soil fungi for their evaluation against the plant parasitic nematode Nacobbus aberrans.

机构信息

CONICET - Laboratorio de Ecología Microbiana, Universidad Nacional de Río Cuarto (UNRC), Río Cuarto, Argentina.

CNR - Istituto per la Protezione Sostenibile delle Piante, Sede di Bari, via G. Amendola, 122/D, 70126, Bari, Italy.

出版信息

World J Microbiol Biotechnol. 2018 Apr 17;34(5):63. doi: 10.1007/s11274-018-2441-8.

DOI:10.1007/s11274-018-2441-8
PMID:29666935
Abstract

The plant-parasitic nematode Nacobbus aberrans is an endoparasite causing severe losses to a wide range of crops from North to South America. The use of native antagonistic fungi may be considered as a possible biological control alternative to reduce the damages caused by this species. Antagonistic effects of 66 potential nematophagous fungi against eggs (J1) and second-stage juveniles (J2) of N. aberrans, were evaluated in vitro on water agar. DGC test showed significant differences (p < 0.0001) in the efficacy of some fungal isolates tested, with parasitism levels for J1 and J2 of 0-95 and 1-78%, respectively. Five isolates of Purpureocillium lilacinum, Metarhizium robertsii and Plectosphaerella plurivora appeared as the most effective antagonists of N. aberrans, relying on hyphae and adhesive conidia in host infection processes.

摘要

植物寄生线虫 Nacobbus aberrans 是一种内寄生线虫,对从北美洲到南美洲的广泛作物造成严重损失。利用本地拮抗真菌可能被视为一种减少该物种造成损害的可行生物防治替代方法。在水琼脂上对 66 种潜在的食线虫真菌对 N. aberrans 的卵 (J1) 和第二龄幼虫 (J2) 的拮抗作用进行了体外评估。DGC 测试显示,一些测试真菌分离物的功效存在显著差异(p<0.0001),J1 和 J2 的寄生率分别为 0-95%和 1-78%。Purpureocillium lilacinum、Metarhizium robertsii 和 Plectosphaerella plurivora 的 5 个分离物在宿主感染过程中依靠菌丝和粘性分生孢子,表现出对 N. aberrans 的最高拮抗作用。

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