人乳头瘤病毒：鼻窦内翻性乳头状瘤中一个不太可能的病因因素。
Human papillomavirus: An unlikely etiologic factor in sinonasal inverted papilloma.
作者信息
Mohajeri Sepideh, Lai Chi, Purgina Bibianna, Almutairi Dakheelallah, Baghai Tabassom, Dimitroulakos Jim, Kilty Shaun
机构信息
Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Department of Pathology, University of Ottawa, Ottawa, Ontario, Canada.
出版信息
Laryngoscope. 2018 Nov;128(11):2443-2447. doi: 10.1002/lary.27207. Epub 2018 Apr 18.
OBJECTIVES/HYPOTHESIS: Tenuous evidence has supported the hypothesis that sinonasal inverted papilloma (SNIP) arise from human papillomavirus (HPV) infection. To clarify the role of HPV in SNIP, all known HPV sub-types were evaluated by employing a robust polymerase chain reaction-based method in a wide variety of SNIPs from a single institution.
STUDY DESIGN
Retrospective surgical specimen tumor sample analysis.
METHODS
HPV positivity among SNIP samples and those with squamous cell carcinoma (SCC) were compared. Immunohistochemistry was used to quantify p16 (over)expression among tumors as a surrogate marker for HPV.
RESULTS
HPV was detected in 10/76 (13%) SNIP specimens. Identified HPV subtypes included nononcogenic 6 and 11 (6/76, 8%) and oncogenic 16, 18, 45, 56 (4/76, 5%). There was no HPV positivity among SCC samples. Only 4/10 (40%) HPV + samples had > 75% p16 cell staining.
CONCLUSION
HPV is not supported as an etiological driver of SNIP development or progression to SCC. The p16 biomarker is not a sensitive indicator of HPV positivity in SNIP.
LEVEL OF EVIDENCE
NA Laryngoscope, 2443-2447, 2018.
目的/假设：已有微弱证据支持鼻窦内翻性乳头状瘤（SNIP）由人乳头瘤病毒（HPV）感染引起这一假说。为阐明HPV在SNIP中的作用，采用一种基于聚合酶链反应的可靠方法，对来自单一机构的多种SNIP样本中的所有已知HPV亚型进行了评估。
研究设计
回顾性手术标本肿瘤样本分析。
方法
比较SNIP样本与鳞状细胞癌（SCC）样本中的HPV阳性情况。免疫组织化学用于量化肿瘤中p16（过）表达，作为HPV的替代标志物。
结果
在76份SNIP标本中的10份（13%）检测到HPV。鉴定出的HPV亚型包括非致癌性的6型和11型（76份中的6份，8%）以及致癌性的16型、18型、45型、56型（76份中的4份，5%）。SCC样本中未检测到HPV阳性。仅4/10（40%）的HPV阳性样本p16细胞染色>75%。
结论
不支持HPV作为SNIP发生或进展为SCC的病因驱动因素。p16生物标志物不是SNIP中HPV阳性的敏感指标。
证据水平
NA 《喉镜》，2018年，第2443 - 2447页
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