Helgeland K, Leirskar J
Scand J Dent Res. 1976 Jan;84(1):37-45. doi: 10.1111/j.1600-0722.1976.tb00459.x.
To investigate the mechanism for the toxicity of silicate cement as observed in a cell culture system, the effects of pH and fluoride were tested on human epithelial cells (NCTC 2544). At pH 7.3, fluoride concentrations from 15 to 25 mug/ml (0.79 to 1.3 mM) had a growth inhibitory effect. When pH of the incubation medium was lowered in the range 7.0 to 6.4, an enhanced cytoxic effect of fluoride was found, and even at 5 to 10 mug/ml growth inhibition occurred. Concomitant with the enhanced cytotoxicity of fluoride at low pH, there was an increased utilization of glucose and formation of lactate. Upon lowering the pH of the incubation medium from 7.4 to 6.7 a twofold increase in the intracellular concentration of fluoride was found.
为研究在细胞培养系统中观察到的硅酸盐水泥毒性机制，测试了pH值和氟化物对人上皮细胞（NCTC 2544）的影响。在pH 7.3时，15至25微克/毫升（0.79至1.3毫摩尔）的氟化物浓度具有生长抑制作用。当孵育培养基的pH值降至7.0至6.4范围内时，发现氟化物的细胞毒性作用增强，即使在5至10微克/毫升时也会发生生长抑制。在低pH值下，随着氟化物细胞毒性的增强，葡萄糖的利用率增加，乳酸生成增加。将孵育培养基的pH值从7.4降至6.7时，细胞内氟化物浓度增加了两倍。
