Li Xiong, Wu Yuansheng, Li Boqun, He Wenqi, Yang Yonghong, Yang Yongping
Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
China Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
Front Genet. 2018 Apr 4;9:103. doi: 10.3389/fgene.2018.00103. eCollection 2018.
The cation diffusion facilitator (CDF) family is one of the gene families involved in metal ion uptake and transport in plants, but the understanding of the definite roles and mechanisms of most genes remain limited. In the present study, we identified 18 candidate genes from the turnip genome and named them -. Then, we performed a comparative genomic analysis on the phylogenetic relationships, gene structures and chromosome distributions, conserved domains, and motifs of turnip CDFs. The constructed phylogenetic tree indicated that the BrrMTPs were divided into seven groups (groups 1, 5, 6, 7, 8, 9, and 12) and formed three major clusters (Zn-CDFs, Fe/Zn-CDFs, and Mn-CDFs). Moreover, the structural characteristics of the BrrMTP members in the same group were similar but varied among groups. To investigate the potential roles of BrrMTPs in turnip, we conducted an expression analysis on all genes under Mg, Zn, Cu, Mn, Fe, Co, Na, and Cd stresses. Results showed that the expression levels of all members were induced by at least one metal ion, indicating that these genes may be related to the tolerance or transport of those metal ions. Based on the roles of different metal ions for plants, we hypothesized that genes are possibly involved in heavy metal accumulation and tolerance to salt stress apart from their roles in the maintenance of mineral nutrient homeostasis in turnip. These findings are helpful to understand the roles of MTPs in plants and provide preliminary information for the study of the functions of genes.
阳离子扩散促进因子（CDF）家族是参与植物金属离子吸收和转运的基因家族之一，但对大多数基因的确切作用和机制的了解仍然有限。在本研究中，我们从芜菁基因组中鉴定出18个候选基因并将它们命名为-。然后，我们对芜菁CDF的系统发育关系、基因结构和染色体分布、保守结构域和基序进行了比较基因组分析。构建的系统发育树表明，BrrMTPs被分为七组（第1、5、6、7、8、9和12组）并形成三个主要簇（锌-CDFs、铁/锌-CDFs和锰-CDFs）。此外，同一组中BrrMTP成员的结构特征相似，但组间有所不同。为了研究BrrMTPs在芜菁中的潜在作用，我们对所有基因在镁、锌、铜、锰、铁、钴、钠和镉胁迫下进行了表达分析。结果表明，所有成员的表达水平至少被一种金属离子诱导，表明这些基因可能与那些金属离子的耐受性或转运有关。基于不同金属离子对植物的作用，我们推测基因除了在维持芜菁矿质营养稳态中的作用外，可能还参与重金属积累和对盐胁迫的耐受性。这些发现有助于了解MTPs在植物中的作用，并为研究基因的功能提供初步信息。
