Ma Jin-Qi, Jian Hong-Ju, Yang Bo, Lu Kun, Zhang Ao-Xiang, Liu Pu, Li Jia-Na
College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
Gene. 2017 Jul 15;620:36-45. doi: 10.1016/j.gene.2017.03.030. Epub 2017 Mar 29.
Growth regulating-factors (GRFs) are plant-specific transcription factors that help regulate plant growth and development. Genome-wide identification and evolutionary analyses of GRF gene families have been performed in Arabidopsis thaliana, Zea mays, Oryza sativa, and Brassica rapa, but a comprehensive analysis of the GRF gene family in oilseed rape (Brassica napus) has not yet been reported. In the current study, we identified 35 members of the BnGRF family in B. napus. We analyzed the chromosomal distribution, phylogenetic relationships (Bayesian Inference and Neighbor Joining method), gene structures, and motifs of the BnGRF family members, as well as the cis-acting regulatory elements in their promoters. We also analyzed the expression patterns of 15 randomly selected BnGRF genes in various tissues and in plant varieties with different harvest indices and gibberellic acid (GA) responses. The expression levels of BnGRFs under GA treatment suggested the presence of possible negative feedback regulation. The evolutionary patterns and expression profiles of BnGRFs uncovered in this study increase our understanding of the important roles played by these genes in oilseed rape.
生长调节因子（GRFs）是植物特有的转录因子，有助于调控植物的生长和发育。已在拟南芥、玉米、水稻和白菜中对GRF基因家族进行了全基因组鉴定和进化分析，但尚未见关于油菜（甘蓝型油菜）GRF基因家族的全面分析报道。在本研究中，我们在甘蓝型油菜中鉴定出35个BnGRF家族成员。我们分析了BnGRF家族成员的染色体分布、系统发育关系（贝叶斯推断和邻接法）、基因结构和基序，以及它们启动子中的顺式作用调控元件。我们还分析了15个随机选择的BnGRF基因在不同组织以及具有不同收获指数和赤霉素（GA）反应的植物品种中的表达模式。GA处理下BnGRFs的表达水平表明可能存在负反馈调节。本研究中揭示的BnGRFs的进化模式和表达谱增进了我们对这些基因在油菜中所起重要作用的理解。
