Zeballos Rafael, Bologna-Molina Ronell, Pereira-Prado Vanesa, Villarroel-Dorrego Mariana
Hospital General del Oeste, Caracas, Venezuela.
Molecular Pathology Area, Faculty of Dentistry, Universidad de la República, Uruguay.
J Clin Exp Dent. 2018 Feb 1;10(2):e172-e176. doi: 10.4317/jced.54222. eCollection 2018 Feb.
Presence of parathyroid hormone related protein (PTHrP) might suggest that ameloblastomas recapitulate features of the enamel epithelium and induce bone resorption, which would facilitate their growth and local invasion. The aim of this study was to determine the expression of PTHrP in ameloblastomas.
An observational research study was designed including 39 cases of histologically diagnosed ameloblastomas (39 out of 42 patients gave consent for the use of their medical records and all data required for this study). Gender, age, tumor location, histological type and subtype of the tumor were recorded and PTHrP expression was determined by indirect immunohistochemistry using monoclonal anti-human PTHrP (1D1 / Santa Cruz Biotechnology). Protein expression and intensity were evaluated under light microscope and finally data recorded and statistically analyzed. This research was approved by the Caracas West General Hospital review board.
39 cases of ameloblastomas were evenly distributed between genders (49% male and 51% female) with a mean age of 33 ± 3.53 years, mainly affecting the posterior mandible. 20 cases (51.28%) showed positive cytoplasmic immunoreactivity to PTHrP. 8 out of 15 cases of solid/multicystic ameloblastomas and 12 out of 23 cases of unicystic ameloblastomas were PTHrP positive. Intense expression of PTHrP was observed in 4 unicystic ameloblastomas (all luminal subtype) and in 5 cases of conventional ameloblastomas.
In the present study PTHrP expression in solid multicystic and unicystic ameloblastoma suggests its possible function in the biological behavior of the tumor. More studies are needed in order to determine the possible role of this protein related to bone invasion processes. Parathyroid hormone related protein, PTHrP, ameloblastoma, bone.
甲状旁腺激素相关蛋白（PTHrP）的存在可能表明成釉细胞瘤重现了釉质上皮的特征并诱导骨吸收，这将促进其生长和局部侵袭。本研究的目的是确定PTHrP在成釉细胞瘤中的表达。
设计了一项观察性研究，纳入39例经组织学诊断的成釉细胞瘤（42例患者中有39例同意使用其病历及本研究所需的所有数据）。记录患者的性别、年龄、肿瘤位置、肿瘤的组织学类型和亚型，并使用单克隆抗人PTHrP（1D1 / 圣克鲁斯生物技术公司）通过间接免疫组织化学法测定PTHrP表达。在光学显微镜下评估蛋白表达和强度，最后记录数据并进行统计分析。本研究经加拉加斯西部综合医院审查委员会批准。
39例成釉细胞瘤在性别上分布均匀（男性49%，女性51%），平均年龄为33±3.53岁，主要累及下颌骨后部。20例（51.28%）显示对PTHrP呈阳性细胞质免疫反应。15例实性/多囊性成釉细胞瘤中有8例，23例单囊性成釉细胞瘤中有12例PTHrP呈阳性。在4例单囊性成釉细胞瘤（均为管腔内亚型）和5例传统成釉细胞瘤中观察到PTHrP的强烈表达。
在本研究中，实性多囊性和单囊性成釉细胞瘤中PTHrP的表达表明其在肿瘤生物学行为中可能发挥的作用。需要更多研究以确定该蛋白与骨侵袭过程相关的可能作用。甲状旁腺激素相关蛋白、PTHrP、成釉细胞瘤、骨。
