Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
Molecules. 2018 Apr 17;23(4):929. doi: 10.3390/molecules23040929.
The gene catalyzes the conversion of diene fatty acids to triene fatty acids and is a key enzyme that determines the synthesis of alpha-linolenic acid. In this study, the full-length cDNAs of , , and are cloned from T. Hong & J. X. Zhang and named as , , and . Their open reading frame is 1203 bp, 1152 bp, and 1353 bp which encoded 400, 371, and 450 amino acids. The molecular weights of the amino acids are 46 kDa, 43 kDa, and 51 kDa while the isoelectric points are 7.34, 8.74, and 9.23, respectively. Bioinformatics analysis shows that all three genes are hydrophobic-hydrophobic, has three transmembrane domains, and and have two transmembrane domains. Multiple series alignment and phylogenetic analysis revealed that and are closely related while is more closely related to . Subcellular localization results showed that was located on the ER membrane and and were located on the chloroplast membrane. The relative expression level of in seeds is very high. expressed more in the ovary than the other two genes. was highly expressed in roots, stems, leaves, petals, and ovaries.
该基因催化二烯脂肪酸向三烯脂肪酸的转化，是决定α-亚麻酸合成的关键酶。本研究从 T. Hong & J. X. Zhang 中克隆了全长 cDNA ，并分别命名为 、 、 。它们的开放阅读框分别为 1203bp、1152bp 和 1353bp，分别编码 400、371 和 450 个氨基酸。氨基酸的分子量分别为 46kDa、43kDa 和 51kDa，等电点分别为 7.34、8.74 和 9.23。生物信息学分析表明，这三个基因均为疏水性-疏水性， 具有三个跨膜结构域， 和 具有两个跨膜结构域。多序列比对和系统发育分析表明 和 密切相关， 与 更为密切相关。亚细胞定位结果表明 定位于内质网膜上， 和 定位于叶绿体膜上。 在种子中的相对表达水平非常高。 在卵巢中的表达量高于其他两个基因。 在根、茎、叶、花瓣和卵巢中均有较高表达。
