Chen Nan, He Sai, Geng Jie, Song Zhang-Jun, Han Pi-Hua, Qin Juan, Zhao Zheng, Song Yong-Chun, Wang Hu-Xia, Dang Cheng-Xue
Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Department of Breast Cancer, Shaanxi Provincial Tumor Hospital, Xi'an, Shaanxi, China.
BMC Cell Biol. 2018 Apr 19;19(1):5. doi: 10.1186/s12860-018-0154-3.
Contactin1 (CNTN1) has been shown to play an important role in the invasion and metastasis of several tumors; however, the role of CNTN1 in breast cancer has not been fully studied. The purpose of this study is to investigate the role of CNTN1 in regulating tumor growth, migration and invasion in breast cancer.
To investigate its function, CNTN1 was expressed in Hs578T cells. CNTN1 expression was confirmed by western blot, immunohistochemistry and real-time RT-PCR. The effect of CNTN1 overexpression on proliferation, migration and invasion of Hs578T breast cancer cells was assessed in vitro and in vivo. Our results showed that CNTN1 overexpression promoted Hs578T cell proliferation, cell cycle progression, colony formation, invasion and migration. Notably, overexpression of CNTN1 in Hs578T cells enhanced the growth of mouse xenograft tumors.
CNTN1 promotes growth, metastasis and invasion of Hs578T breast cancer cell line. Thus, therapies targeting CNTN1 may prove efficacious for breast cancer. However, further investigation is required to understand the mechanism by which CNTN1 influences proliferation, metastasis and invasion in breast cancer.
Contactin1（CNTN1）已被证明在多种肿瘤的侵袭和转移中起重要作用；然而，CNTN1在乳腺癌中的作用尚未得到充分研究。本研究的目的是探讨CNTN1在调节乳腺癌肿瘤生长、迁移和侵袭中的作用。
为研究其功能，在Hs578T细胞中表达了CNTN1。通过蛋白质免疫印迹、免疫组织化学和实时逆转录聚合酶链反应证实了CNTN1的表达。在体外和体内评估了CNTN1过表达对Hs578T乳腺癌细胞增殖、迁移和侵袭的影响。我们的结果表明，CNTN1过表达促进了Hs578T细胞增殖、细胞周期进程、集落形成、侵袭和迁移。值得注意的是，Hs578T细胞中CNTN1的过表达增强了小鼠异种移植肿瘤的生长。
CNTN1促进Hs578T乳腺癌细胞系的生长、转移和侵袭。因此，针对CNTN1的治疗可能对乳腺癌有效。然而，需要进一步研究以了解CNTN1影响乳腺癌增殖、转移和侵袭的机制。
