外泌体传递的 miR-93-5p 通过靶向 PTEN 促进食管癌细胞的增殖和细胞间通讯。

miR-93-5p Transferred by Exosomes Promotes the Proliferation of Esophageal Cancer Cells via Intercellular Communication by Targeting PTEN.

机构信息

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China.

Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, Jiangsu, China; Hangzhou First People's Hospital, Hangzhou 310000, Zhejiang, China.

出版信息

Biomed Environ Sci. 2018 Mar;31(3):171-185. doi: 10.3967/bes2018.023.

DOI:10.3967/bes2018.023

PMID:29673440

Abstract

OBJECTIVE

To investigate the relationship between plasma miR-93-5p and the risk of esophageal cancer, as well as the influence of miR-93-5p on the biological function of esophageal cancer cells, exerted through exosomes.

METHODS

The expression of plasma miR-93-5p in esophageal cancer patients and healthy controls was analysed by real-time quantitative PCR. The influence of miR-93-5p on the risk and prognosis of esophageal carcinoma was analyzed by conditional logistic regression and survival analysis. The effect of miR-93-5p on the biological function of recipient cells was investigated by establishing an in vitro donor cell co-culture model. The target gene of miR-93-5p was validated by luciferase reporter assay and Western Blotting.

RESULTS

Upregulation of plasma miR-93-5p expression significantly increases the risk of esophageal cancer and is associated with poor prognosis. miR-93-5p transferred by exosomes promotes the proliferation of recipient esophageal cancer cells and affects the expression of PTEN and its downstream proteins p21 and cyclin D1.

CONCLUSION

Our study provides a reference for the identification of biomarkers for the diagnosis and prognosis of esophageal cancer.

摘要

目的

研究血浆 miR-93-5p 与食管癌风险的关系，以及 miR-93-5p 通过外泌体对食管癌细胞生物学功能的影响。

方法

采用实时定量 PCR 分析食管癌患者和健康对照者血浆 miR-93-5p 的表达。采用条件 logistic 回归和生存分析分析 miR-93-5p 对食管癌风险和预后的影响。通过建立体外供体细胞共培养模型，研究 miR-93-5p 对受体细胞生物学功能的影响。通过荧光素酶报告基因检测和 Western Blotting 验证 miR-93-5p 的靶基因。