Laboratory of Environmental Analytical Chemistry-LQA2, University of the Balearic Islands, Cra.Valldemossa km 7.5, 07122 Palma de Mallorca, Spain; Environment and Energy Department, Advanced Materials Research Center, Miguel de Cervantes 120, 31136 Chihuahua, Mexico.
Laboratory of Environmental Analytical Chemistry-LQA2, University of the Balearic Islands, Cra.Valldemossa km 7.5, 07122 Palma de Mallorca, Spain.
Talanta. 2018 Jul 1;184:15-22. doi: 10.1016/j.talanta.2018.02.065. Epub 2018 Feb 16.
A 3D printed device for the fully automated disk-based solid-phase extraction (SPE) of Cr (VI) from water samples has been fabricated. The compatibility of the use of organic solvents for analyte elution with 3D printed flow devices based on polymers fabricated using stereolithography has been evaluated. The developed methodology comprises the complexation of Cr (VI) with 1, 5-diphenylcarbazide (DPC) in acidic medium and the subsequent retention of the complex in a SBD-RPS disk contained within the 3D printed device. A multisyringe flow injection analysis system with online spectrophotometric detection has been used for the automation of the method. The fabricated 3D printed device integrates the different components of the flow analysis manifold, including connectors and mixers, being a powerful approach towards the reproducible construction of highly integrated flow-based manifolds. The extracted Cr (VI)-DPC complex is eluted with a mixture of methanol- sulfuric acid and quantified at 540 nm. The effect on the analytical signal and the optimization of variables were evaluated using multivariate and univariate techniques. A detection limit of 1 ng Cr (VI) and a linear working range of 3.2-600 ng Cr (VI) were obtained using a sample volume of 2 ml. The intra-day and inter-day RSDs were 4.8% (10 µg L, n = 12) and 3.4% (n = 5, different day with a different disk), respectively. The applicability of the fabricated 3D printed device has been proved by the determination of Cr (VI) in groundwater, surface water and leachates.
已制备出一种用于全自动基于磁盘的固相萃取（SPE）水样中六价铬（Cr（VI））的 3D 打印设备。评估了使用有机溶剂洗脱分析物与使用立体光刻技术制造的基于聚合物的 3D 打印流道装置的兼容性。所开发的方法包括在酸性介质中用 1,5-二苯卡巴肼（DPC）络合 Cr（VI），以及随后将络合物保留在 3D 打印装置内的 SBD-RPS 磁盘中。采用多注射器流动注射分析系统和在线分光光度检测法实现了方法的自动化。所制备的 3D 打印设备集成了流分析歧管的不同组件，包括连接器和混合器，是实现高度集成的基于流的歧管可重现构建的强大方法。用甲醇-硫酸混合物洗脱提取的 Cr（VI）-DPC 络合物，并在 540nm 处定量。使用多元和单变量技术评估了对分析信号的影响并优化了变量。使用 2ml 样品体积，可获得 1ng Cr（VI）的检测限和 3.2-600ng Cr（VI）的线性工作范围。日内和日间 RSD 分别为 4.8%（10μg L，n=12）和 3.4%（n=5，不同天使用不同的磁盘）。通过测定地下水、地表水和浸出液中的 Cr（VI），证明了所制备的 3D 打印设备的适用性。
