Centre for Nuclear Energy in Agriculture, University of Sao Paulo, Av. Centenário 303, P.O. Box 96, 13400-970 Piracicaba, SP, Brazil.
Luiz de Queiroz College of Agriculture, University of São Paulo, P.O. Box 9, 13418-970 Piracicaba, SP, Brazil.
Talanta. 2018 Jul 1;184:325-331. doi: 10.1016/j.talanta.2018.02.072. Epub 2018 Feb 21.
In flow analysis, solid particles (sorbents, reagents or catalysts) have been used for e.g. analyte separation/concentration, sample clean-up, speciation analysis, enzymatic assays, analysis relying on slight soluble reagents, and kinetics studies related to adsorption/release of species. The particles are usually accommodated inside packed-bed mini-columns, cartridges or disks, but this geometry may led to limited analyte/particle interaction, poor renewal of the particle surface, swelling effects, establishment of preferential pathways, and increased backpressure. These hindrances are circumvented by fluidizing the solid particles. Fluidization is a worldwide-accepted industrial process, which can be successfully implemented in flow analysis. This review emphasizes historical and conceptual aspects, as well as advantages, limitations, applications, and perspectives for future development of flow analysis relying on fluidized particles.
在流动分析中，固体颗粒（吸附剂、试剂或催化剂）已被用于例如分析物的分离/浓缩、样品净化、形态分析、酶分析、依赖于微溶性试剂的分析以及与物种吸附/释放相关的动力学研究。这些颗粒通常被容纳在填充床微型柱、试剂盒或磁盘内，但这种几何形状可能会导致分析物与颗粒之间的相互作用有限、颗粒表面的更新不良、溶胀效应、优先途径的建立以及背压增加。通过流化固体颗粒可以避免这些障碍。流化是一种被广泛接受的工业过程，可以成功地应用于流动分析中。本综述强调了基于流化颗粒的流动分析的历史和概念方面，以及优点、局限性、应用和未来发展的展望。
