Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, 4-101 Koyama-Minami, Tottori, 680-8552, Japan.
Institute of Biomedical Sciences and Graduate School of Pharmaceutical Sciences, Tokushima University, Shomachi, Tokushima, 770-8505, Japan.
Sci Rep. 2018 Apr 19;8(1):6243. doi: 10.1038/s41598-018-24675-7.
Light-driven nano/micromotors are attracting much attention, not only as molecular devices but also as components of bioinspired robots. In nature, several pathogens such as Listeria use actin polymerisation machinery for their propulsion. Despite the development of various motors, it remains challenging to mimic natural systems to create artificial motors propelled by fibre formation. Herein, we report the propulsion of giant liposomes driven by light-induced peptide nanofibre growth on their surface. Peptide-DNA conjugates connected by a photocleavage unit were asymmetrically introduced onto phase-separated giant liposomes. Ultraviolet (UV) light irradiation cleaved the conjugates and released peptide units, which self-assembled into nanofibres, driving the translational movement of the liposomes. The velocity of the liposomes reflected the rates of the photocleavage reaction and subsequent fibre formation of the peptide-DNA conjugates. These results showed that chemical design of the light-induced peptide nanofibre formation is a useful approach to fabricating bioinspired motors with controllable motility.
光驱动的纳米/微米马达备受关注，不仅可用作分子器件，还可用作仿生机器人的组件。在自然界中，李斯特菌等几种病原体利用肌动蛋白聚合机制来推动自身运动。尽管已经开发出了各种马达，但要模仿自然系统来创建由纤维形成驱动的人工马达仍然具有挑战性。在此，我们报告了在其表面的光诱导肽纳米纤维生长的驱动下，巨型脂质体的推进。通过光裂解单元连接的肽-DNA 缀合物不对称地引入到相分离的巨型脂质体中。紫外线 (UV) 光照射使缀合物裂解，并释放出肽单元，这些单元自组装成纳米纤维，驱动脂质体的平移运动。脂质体的速度反映了光裂解反应和随后的肽-DNA 缀合物的纤维形成的速率。这些结果表明，光诱导肽纳米纤维形成的化学设计是一种制造具有可控运动性的仿生马达的有效方法。
