Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, China.
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Haebin, China.
Sci Rep. 2018 Apr 19;8(1):6255. doi: 10.1038/s41598-018-24562-1.
The Amur sturgeon, Acipenser schrenckii, is an important aquaculture species in China with annual production of about 150 thousand tons in 2015. In this study, we investigated the regulatory proteins and pathways affecting embryonic development of Amur sturgeon, by analyzing of the differential proteomes among four embryonic developmental stages using isobaric tags for relative and absolute quantitation (iTRAQ), combined with the analysis of effects of microelements and antioxidants on embryonic development. Seventy-four, 77, and 76 proteins were differentially expressed according to iTRAQ analysis between the fertilized egg and blastula, blastula and neurula, and neurula and heart-beat stages, respectively. GO and KEGG enrichment analyses indicated that Gluconeogenesis, Ribosome and Proteasome were the most enriched pathways, which may promote energy formation, immune system protection and protein synthesis process in A. schrenckii. The measurement of microelements indicated that Mn, Cu and Fe were obtained from their parents or water environment in A. schrenckii, while Zn plays vital roles throughout embryonic development. The dramatically high level of malondialdehyde (MDA) across the embryonic development may be the main reason leading to a low hatching rate in A. schrenckii. This study provides the basis for further proteome analysis of embryonic development in A. schrenckii.
达乌尔鳇，施氏鲟，是中国重要的水产养殖品种，2015 年的年产量约为 15 万吨。在这项研究中，我们通过使用同位素相对和绝对定量标记（iTRAQ）分析四个胚胎发育阶段之间的差异蛋白质组，结合微量元素和抗氧化剂对胚胎发育的影响分析，研究了影响达乌尔鳇胚胎发育的调节蛋白和途径。根据 iTRAQ 分析，在受精卵和囊胚、囊胚和神经胚以及神经胚和心跳阶段之间，分别有 74、77 和 76 种蛋白质差异表达。GO 和 KEGG 富集分析表明，糖异生、核糖体和蛋白酶体是最丰富的途径，这可能促进了达乌尔鳇的能量形成、免疫系统保护和蛋白质合成过程。微量元素的测量表明，Mn、Cu 和 Fe 是从其父母或水环境中获得的，而 Zn 在整个胚胎发育过程中起着至关重要的作用。胚胎发育过程中丙二醛（MDA）水平的急剧升高可能是导致达乌尔鳇孵化率低的主要原因。本研究为进一步分析达乌尔鳇胚胎发育的蛋白质组提供了基础。
