Teodoro Guilherme R, Gontijo Aline V L, Salvador Marcos J, Tanaka Márcia H, Brighenti Fernanda L, Delbem Alberto C B, Delbem Ádina C B, Koga-Ito Cristiane Y
Environmental Engineering Department and Oral Biopathology Graduate Program, Institute of Science and Technology of São José dos Campos, São Paulo State University, São Paulo, Brazil.
Department of Plant Biology, PPGBTPB, Institute of Biology, University of Campinas, Campinas, Brazil.
Front Microbiol. 2018 Apr 5;9:647. doi: 10.3389/fmicb.2018.00647. eCollection 2018.
A promising anti- activity of extracts was recently described. In the present work, experiments were carried out to determine the fraction with higher antifungal activity from a extract. Acetone fraction (AF) was obtained from the aqueous extract from dried leaves (5 min/100°C) and it was the most effective one. Gallic acid (GA) was identified by electrospray ionization mass spectrometry (ESI-MS) and also chosen to perform antifungal tests due to its promising activity on . Minimal inhibitory and fungicidal concentrations (MIC and MFC) were determined by broth microdilution technique. The effect on virulence factors of was evaluated, and the cytotoxicity was determined. MIC and MIC values were both equal to 0.625 mg ml for AF and 2.5 and 5 mg ml, respectively, for GA. AF and GA showed ability to inhibit adherence and to disrupt 48 h-biofilm. AF and GA were effective in reducing the formation of hyphae of SC5314. AF and GA decreased adherence of to oral epithelial cells. AF and GA showed slight to moderate toxicity to Vero cells. This result suggests further studies for topic use of these compounds. AF, which contains a combination of several molecules, presented greater potential of antimicrobial activity than GA, with lower values of MIC and lower cytoxicity.
最近报道了提取物有良好的抗活性。在本研究中，开展实验以从一种提取物中确定具有更高抗真菌活性的组分。丙酮组分（AF）是从干叶水提取物（100°C/5分钟）中获得的，它是最有效的组分。通过电喷雾电离质谱（ESI-MS）鉴定出没食子酸（GA），并且由于其对……有良好活性也选择其进行抗真菌试验。采用肉汤微量稀释技术测定最小抑菌浓度和杀菌浓度（MIC和MFC）。评估了对……毒力因子的影响，并测定了细胞毒性。AF的MIC和MFC值均等于0.625毫克/毫升，GA的MIC和MFC值分别为2.5毫克/毫升和5毫克/毫升。AF和GA显示出抑制……黏附以及破坏48小时生物膜的能力。AF和GA可有效减少白色念珠菌SC5314菌丝的形成。AF和GA降低了白色念珠菌对口腔上皮细胞的黏附。AF和GA对Vero细胞显示出轻度至中度毒性。该结果表明对这些化合物的局部应用需要进一步研究。含有多种分子组合的AF比GA具有更大的抗菌活性潜力，其MIC值更低且细胞毒性更小。
