Maljurić Nevena, Golubović Jelena, Ravnikar Matjaž, Žigon Dušan, Štrukelj Borut, Otašević Biljana
Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, Belgrade 11221, Serbia.
Chair for Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Aškerčeva cesta 7, Ljubljana 1000, Slovenia.
J Anal Methods Chem. 2018 Feb 20;2018:2434691. doi: 10.1155/2018/2434691. eCollection 2018.
Diabetes mellitus is one of the leading world's public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of , responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.
糖尿病是全球主要的公共卫生问题之一。因此，开发新型抗糖尿病药物具有极大的意义。除了传统的糖尿病治疗方法外，如今，具有抗糖尿病潜力的天然物质也受到了重视。[某种蘑菇名称]是一种因其已被证实的多种活性而被广泛用于不同用途的蘑菇。烟曲霉醇是[某种蘑菇名称]的一种成分，具有抗糖尿病潜力。在这方面，开发一种从真菌材料中分离和定量烟曲霉醇的简单高效方法非常重要。乙醇避免了先前报道的研究中采用的多步复杂提取方法，实现了快速单步提取。通过高分辨率质谱法确认了乙醇提取物中烟曲霉醇的存在。开发了半制备高效液相色谱法，用于从乙醇提取物中分离并纯化活性化合物烟曲霉醇。这是一种梯度反相方法，流动相由乙腈和0.1%甲酸水溶液组成，总运行时间为15分钟。以甲苯为内标，通过定量核磁共振法测定出6.5毫克高纯度烟曲霉醇。分离并测定的物质含量可进一步用于烟曲霉醇活性的定量评估。
