Park Young-Mi, Kim In-Tae, Park Hee-Juhn, Choi Jong-Won, Park Kun-Young, Lee Jae-Dong, Nam Byung-Hyouk, Kim Deog-Gon, Lee Jin-Yong, Lee Kyung-Tae
College of Pharmacy, Kyung Hee University, Hoegi-Dong, Seoul, Korea.
Biol Pharm Bull. 2004 Oct;27(10):1588-93. doi: 10.1248/bpb.27.1588.
In an attempt to find bioactive natural products with an anti-inflammatory activity, we evaluated the effects of the methanol extract of Fomes fomentarius (MEFF) on in vivo anti-inflammatory and anti-nociceptive activities. MEFF (50, 100 mg/kg/d, p.o.) reduced acute paw edema induced by carrageenin in rats, and showed MEFF analgesic activity, as determined by an acetic acid-induced writhing test and a hot plate test in mice. To investigate the mechanism of the anti-inflammatory action of MEFF, we examined the effect of MEFF on lipopolysaccharide (LPS)-induced responses in murine macrophages cell line RAW 264.7. MEFF potently inhibited the production of nitric oxide (NO), prostaglandin E2 (PGE2), and tumor necrosis factor-alpha (TNF-alpha) in LPS-stimulated RAW 264.7 macrophages. Consistent with these observations, inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) levels were reduced by MEFF in a dose-dependent manner. Furthermore, MEFF suppressed nuclear factor-kappaB (NF-kappaB) activation in LPS-stimulated RAW 264.7 macrophages. These findings suggest that the anti-inflammatory and anti-nociceptive properties of the methanol extract of MEFF may result from the inhibition of iNOS and COX-2 expression through the down-regulation of NF-kappaB binding activity.
为了寻找具有抗炎活性的生物活性天然产物，我们评估了木蹄层孔菌甲醇提取物（MEFF）对体内抗炎和抗伤害感受活性的影响。MEFF（50、100毫克/千克/天，口服）减轻了角叉菜胶诱导的大鼠急性爪肿胀，并在小鼠的醋酸诱导扭体试验和热板试验中显示出MEFF的镇痛活性。为了研究MEFF抗炎作用的机制，我们检测了MEFF对小鼠巨噬细胞系RAW 264.7中脂多糖（LPS）诱导反应的影响。MEFF强烈抑制LPS刺激的RAW 264.7巨噬细胞中一氧化氮（NO）、前列腺素E2（PGE2）和肿瘤坏死因子-α（TNF-α）的产生。与这些观察结果一致，MEFF以剂量依赖性方式降低了诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的水平。此外，MEFF抑制了LPS刺激的RAW 264.7巨噬细胞中核因子-κB（NF-κB）的激活。这些发现表明，MEFF甲醇提取物的抗炎和抗伤害感受特性可能是通过下调NF-κB结合活性来抑制iNOS和COX-2表达的结果。
