Brooks Wilson C, Paguigan Noemi D, Raja Huzefa A, Moy Franklin J, Cech Nadja B, Pearce Cedric J, Oberlies Nicholas H
Department of Chemistry and Biochemistry, University of North Carolina at Greensboro, PO Box 26170, Greensboro, NC, 27402, USA.
Mycosynthetix, Inc, 505 Meadowland Drive, Suite 103, Hillsborough, NC, 27278, USA.
Magn Reson Chem. 2017 Jul;55(7):670-676. doi: 10.1002/mrc.4571. Epub 2017 Feb 5.
Analysis of complex mixtures is a common challenge in natural products research. Quantitative nuclear magnetic resonance spectroscopy offers analysis of complex mixtures at early stages and with benefits that are orthogonal to more common methods of quantitation, including ultraviolet absorption spectroscopy and mass spectrometry. Several experiments were conducted to construct a methodology for use in analysis of extracts of fungal cultures. A broadly applicable method was sought for analysis of both pure and complex samples through use of an externally calibrated method. This method has the benefit of not contaminating valuable samples with the calibrant, and it passed scrutiny for line fitting and reproducibility. The method was implemented to measure the yield of griseofulvin and dechlorogriseofulvin from three fungal isolates. An isolate of Xylaria cubensis (coded MSX48662) was found to biosynthesize griseofulvin in the greatest yield, 149 ± 8 mg per fermentation, and was selected for further supply experiments. Copyright © 2016 John Wiley & Sons, Ltd.
复杂混合物的分析是天然产物研究中的一项常见挑战。定量核磁共振光谱法能够在早期阶段对复杂混合物进行分析，其优势与更常用的定量方法（包括紫外吸收光谱法和质谱法）互为补充。开展了多项实验以构建一种用于分析真菌培养物提取物的方法。通过使用外部校准方法，寻求一种广泛适用的方法来分析纯样品和复杂样品。该方法的优点是不会用校准剂污染有价值的样品，并且通过了线性拟合和重现性审查。该方法用于测量三种真菌分离株中灰黄霉素和去氯灰黄霉素的产量。发现一种古巴炭角菌分离株（编号为MSX48662）生物合成灰黄霉素的产量最高，每次发酵为149±8毫克，并被选用于进一步的供应实验。版权所有©2016约翰威立父子有限公司。
