Chinwe Gbotolorun Stella, Azuka Okafor Izuchukwu, Adaeze Ndoeche Chidinma
Anatomy Department, College of Medicine of the University of Lagos, Lagos State, Nigeria.
Int J Reprod Biomed. 2018 Jan;16(1):19-30.
Cisplatin is a potent antineoplastic agent for many cancers but causes several levels of gonadal damage. Ovarian toxicity is a major concern of young cancer patients undergoing chemotherapy.
This study sought to examine the effect of Cisplatin and Resveratrol supplementation on ovarian function in Sprague-Dawley rats.
In this experimental study, 45 cyclic Sprague-Dawley rats with an average weight of 160 gr were divided into 9 groups (n=5/group). Group 1 was used as control and received distilled water. Groups 2 and 9 received Cisplatin only. Groups 3, 4, and 5 received different doses of Resveratrol after a single dose of Cisplatin. Groups 6, 7, and 8 received Resveratrol before Cisplatin. At sacrifice, the ovary was analyzed for histopathology, biochemical indices of oxidation and hormonal assay.
Relative and absolute organ weights were notably increased (p=0.001, 0.01) in the prophylactic groups relative to the groups that received Resveratrol after Cisplatin. Also, glutathione, superoxide dismutase and catalase were significantly increased (p=0.047, 0.01, 0.023) in a dose-dependent manner when compared to Cisplatin group only. Malondialdehyde decreased significantly (p=0.001) in the groups that received high dose Resveratrol compared with the control and Cisplatin alone groups. Although oestrogen showed no significant difference within the groups (p=0.48), Resveratrol significantly increased progesterone, follicle stimulating hormone and luteinizing hormone levels (p=0.007, 0.001, 0.006) at high doses when compared with Cisplatin alone groups. Ovarian histoarchitecture was best preserved in the prophylactic groups in a dose-dependent manner.
Resveratrol supplementation confers protection and preserves ovarian follicles from Cisplatin toxicity in Sprague-Dawley rats.
顺铂是一种对多种癌症有效的抗肿瘤药物，但会造成不同程度的性腺损伤。卵巢毒性是接受化疗的年轻癌症患者主要关注的问题。
本研究旨在探讨补充顺铂和白藜芦醇对Sprague-Dawley大鼠卵巢功能的影响。
在本实验研究中，将45只平均体重160克的性周期正常的Sprague-Dawley大鼠分为9组（每组n = 5）。第1组作为对照组，给予蒸馏水。第2组和第9组仅给予顺铂。第3、4和5组在单次给予顺铂后给予不同剂量的白藜芦醇。第6、7和8组在给予顺铂前给予白藜芦醇。处死时，对卵巢进行组织病理学分析、氧化生化指标检测和激素测定。
与顺铂后给予白藜芦醇的组相比，预防组的相对和绝对器官重量显著增加（p = 0.001，0.01）。此外，与仅顺铂组相比，谷胱甘肽、超氧化物歧化酶和过氧化氢酶以剂量依赖方式显著增加（p = 0.047，0. = 0.01，0.023）。与对照组和单独顺铂组相比，高剂量白藜芦醇组的丙二醛显著降低（p = 0.001）。虽然雌激素在各组间无显著差异（p = 0.48），但与单独顺铂组相比，高剂量白藜芦醇显著提高了孕酮、促卵泡激素和促黄体生成素水平（p = 0.007，0.001，0.006）。预防组的卵巢组织结构以剂量依赖方式得到最佳保存。
补充白藜芦醇可保护Sprague-Dawley大鼠的卵巢卵泡免受顺铂毒性的影响。
