Ni Haiyan, Li Na, Qiu Jiguo, Chen Qing, He Jian
College of Life Science, Jiangxi Normal University, Nanchang, 330022, Jiangxi, China.
College of Life Sciences, Laboratory Centre of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Curr Microbiol. 2018 Aug;75(8):1077-1083. doi: 10.1007/s00284-018-1494-0. Epub 2018 Apr 19.
In this study, a bacterial strain P13 capable of degrading pendimethalin was isolated from the soil of a fruit garden. Based on observed cellular morphology and physiology characteristics and a phylogenetic analysis of 16S rRNA gene sequences, strain P13 was identified as a member of the genus Paracoccus. Strain P13 grew on pendimethalin as the sole carbon source, and could degrade 100 mg/L pendimethalin within 2 days and 200 mg/L pendimethalin within 5 days. Pendimethalin degradation was proposed to be initiated by oxidation ring cleavage to yield 1,3-dinitro-2-(pentan-3-ylamino)butane-1,4-diol, an alkane organic compound that was identified by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), which then underwent a series of enzymatic reactions to produce CO and HO. The optimal pH and temperature for pendimethalin degradation by strain P13 were 7.0 and 30 °C, respectively. This study identified the bacterial strain Paracoccus sp. P13, which degraded pendimethalin with a relatively high efficiency, and presents a previously unreported microbial pendimethalin degradation pathway.
在本研究中，从果园土壤中分离出一株能够降解二甲戊灵的细菌菌株P13。基于观察到的细胞形态和生理特征以及16S rRNA基因序列的系统发育分析，菌株P13被鉴定为副球菌属的成员。菌株P13以二甲戊灵作为唯一碳源生长，能够在2天内降解100mg/L的二甲戊灵，并在5天内降解200mg/L的二甲戊灵。据推测，二甲戊灵的降解首先通过氧化开环产生1,3-二硝基-2-(戊-3-基氨基)丁烷-1,4-二醇，这是一种烷烃有机化合物，通过超高效液相色谱-串联质谱法（UHPLC-MS/MS）进行鉴定，然后经过一系列酶促反应生成CO₂和H₂O。菌株P13降解二甲戊灵的最适pH和温度分别为7.0和30℃。本研究鉴定出了高效降解二甲戊灵的副球菌属细菌菌株P13，并提出了一种此前未报道的微生物降解二甲戊灵的途径。
