Xiong Miaohuan, Wu Huipeng, Li Jialu, Wan Yingying, Chen Xuelan, Liu Bin, He Jian
College of Life Sciences, Jiangxi Normal University, Nanchang, 330022, Jiangxi, PR China.
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, PR China.
Curr Microbiol. 2025 Apr 30;82(6):269. doi: 10.1007/s00284-025-04254-6.
A Gram-negative, aerobic, rod-shaped, and non-motile bacterium, designated strain R1, was isolated from lakeside soil in Nanchang, Jiangxi Province, China. Growth was observed at 15-37 °C (optimum 30 °C), pH 6-8 (optimum pH 7) and salinity of 0-1.0% (w/v; optimum 0.5%). Strain R1 completely degraded 5 mg/L of ochratoxin A within 48 h of incubation. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain R1 belongs to the genus Terrimonas, and showed 99% sequences similarity with Terrimonas pollutisoli H1YJ31. The draft genome of strain R1 was approximately 6.2 Mb with a 41.5% G + C content. The average nucleotide identity and digital DNA-DNA hybridization values between strain R1 and T. pollutisoli H1YJ31 were 90.4% and 41.2%, respectively. The predominant fatty acids of strain R1 were iso-C, summed feature 3 (C ω6c and/or C ω7c), iso-C 3-OH and iso-C G. The major isoprenoid quinone was menaquinone 7 (MK-7). The major polar lipids of strain R1 were phosphatidylethanolamine, phosphatidylglycerol, a phospholipid, two unidentified glycolipids, two unidentified aminophospholipids, and five unidentified polar lipids. Based on phenotypic, genotypic and phylogenetic evidence, strain R1 represents a novel species in the genus Terrimonas, for which the name Terrimonas alba sp. nov. is proposed. The type strain is R1 (= GDMCC 1.4673 = JCM 37058 = KCTC 102293 = KACC 23743).
从中国江西省南昌市湖滨土壤中分离出一株革兰氏阴性、需氧、杆状且无运动性的细菌，命名为菌株R1。该菌株在15 - 37°C（最适温度30°C）、pH 6 - 8（最适pH 7）和盐度0 - 1.0%（w/v；最适盐度0.5%）的条件下生长。菌株R1在培养48小时内可完全降解5 mg/L的赭曲霉毒素A。基于16S rRNA基因序列的系统发育分析表明，菌株R1属于地生单胞菌属，与污染地生单胞菌H1YJ31的序列相似性为99%。菌株R1的基因组草图约为6.2 Mb，G + C含量为41.5%。菌株R1与污染地生单胞菌H1YJ31之间的平均核苷酸同一性和数字DNA - DNA杂交值分别为90.4%和41.2%。菌株R1的主要脂肪酸为异C、总和特征3（C ω6c和/或C ω7c）、异C 3 - OH和异C G。主要类异戊二烯醌为甲基萘醌7（MK - 7）。菌株R1的主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、一种磷脂、两种未鉴定的糖脂、两种未鉴定的氨基磷脂和五种未鉴定的极性脂。基于表型、基因型和系统发育证据，菌株R1代表地生单胞菌属中的一个新物种，为此提出名为白色地生单胞菌（Terrimonas alba sp. nov.）的名称。模式菌株为R1（= GDMCC 1.4673 = JCM 37058 = KCTC 102293 = KACC 23743）。
