Centre of Excellence for Advanced Materials and Sensing Devices, Ruđer Bošković Institute, Bijenička cesta 54, 10002 Zagreb, Croatia.
Centre of Excellence for Advanced Materials and Sensing Devices, Ruđer Bošković Institute, Bijenička cesta 54, 10002 Zagreb, Croatia.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Jul 5;200:102-109. doi: 10.1016/j.saa.2018.04.016. Epub 2018 Apr 10.
Modern diagnostic tools ever aim to reduce the amount of analyte and the time needed for obtaining the result. Surface-enhanced Raman spectroscopy is a method that could satisfy both of these requirements, provided that for each analyte an adequate substrate is found. Here we demonstrate the ability of gold-sputtered silicon nanowires (SiNW) to bind p-mercaptobenzoic acid in 10, 10 and 10M and adenine in 30 and 100μM concentrations. Based on the normal mode analysis, presented here for the first time, the binding of p-mercaptobenzoic acid is deduced. The intensity enhancement of the 1106cm band is explained by involvement of the CS stretching deformation, and the appearance of the broad 300cm band attributed to SAu stretching mode. Adenine SERS spectra demonstrate the existence of the 7H tautomer since the strongest band observed is at 736cm. The adenine binding is likely to occur in several ways, because the number of observed bands in the 1200-1600cm interval exceeds the number of observed bands in the normal Raman spectrum of the free molecule.
现代诊断工具旨在减少分析物的数量和获得结果所需的时间。表面增强拉曼光谱是一种可以满足这两个要求的方法，前提是为每种分析物找到合适的基底。在这里，我们展示了镀金硅纳米线（SiNW）在 10、10 和 10M 浓度下结合对巯基苯甲酸和在 30 和 100μM 浓度下结合腺嘌呤的能力。基于这里首次提出的正常模式分析，推断出对巯基苯甲酸的结合。CS 伸缩变形的参与解释了 1106cm 带的强度增强，而归因于 SAu 伸缩模式的宽 300cm 带的出现。腺嘌呤 SERS 光谱表明存在 7H 互变异构体，因为观察到的最强带位于 736cm 处。腺嘌呤的结合可能以多种方式发生，因为在 1200-1600cm 间隔内观察到的带的数量超过了游离分子的正常拉曼光谱中观察到的带的数量。
