Department of Neurology, First Hospital, Jilin University, Changchun 130021, PR China.
Blood Bank, Jilin Women and Children Health Hospital, Changchun 130021, PR China.
Clin Chim Acta. 2018 Aug;483:57-63. doi: 10.1016/j.cca.2018.04.022. Epub 2018 Apr 18.
Tuberculosis meningitis (TBM) is a prevalent form of extra-pulmonary tuberculosis that causes substantial morbidity and mortality. Diagnosis of TBM is difficult because of the limited sensitivity of existing laboratory techniques. A metabolomics approach can be used to investigate the sets of metabolites of both bacteria and host, and has been used to clarify the mechanisms underlying disease development, and identify metabolic changes, leadings to improved methods for diagnosis, treatment, and prognostication. Mass spectrometry (MS) is a major analysis platform used in metabolomics, and MS-based metabolomics provides wide metabolite coverage, because of its high sensitivity, and is useful for the investigation of Mycobacterium tuberculosis (Mtb) and related diseases. It has been used to investigate TBM diagnosis; however, the processes involved in the MS-based metabolomics approach are complex and flexible, and often consist of several steps, and small changes in the methods used can have a huge impact on the final results. Here, the process of MS-based metabolomics is summarized and its applications in Mtb and Mtb-related diseases discussed. Moreover, the current status of TBM metabolomics is described.
结核性脑膜炎（TBM）是一种常见的肺外结核形式，可导致严重的发病率和死亡率。由于现有实验室技术的敏感性有限，TBM 的诊断较为困难。代谢组学方法可用于研究细菌和宿主的代谢物组，并已用于阐明疾病发展的机制，并确定代谢变化，从而改进诊断、治疗和预后的方法。质谱（MS）是代谢组学中主要的分析平台，基于 MS 的代谢组学提供了广泛的代谢物覆盖范围，因为其灵敏度高，适用于结核分枝杆菌（Mtb）和相关疾病的研究。它已被用于研究 TBM 的诊断；然而，基于 MS 的代谢组学方法的过程复杂且灵活，通常包含多个步骤，方法中的微小变化会对最终结果产生巨大影响。在这里，总结了基于 MS 的代谢组学的过程，并讨论了其在 Mtb 和 Mtb 相关疾病中的应用。此外，还描述了 TBM 代谢组学的现状。
