Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
FEBS Lett. 2018 Jun;592(12):1997-2011. doi: 10.1002/1873-3468.13054. Epub 2018 May 2.
Conventional biophysical and chemical biology approaches for delineating relationships between the structure and biological function of nucleic acids (NAs) abstract NAs from their native biological context. However, cumulative experimental observations have revealed that the structure, dynamics and interactions of NAs might be strongly influenced by a broad spectrum of specific and nonspecific physical-chemical environmental factors. This consideration has recently sparked interest in the development of novel tools for structural characterization of NAs in the native cellular context. Here, we review the individual methods currently being employed for structural characterization of NA structure in a native cellular environment with a focus on recent advances and developments in the emerging fields of in-cell NMR and electron paramagnetic resonance spectroscopy and in-cell single-molecule FRET of NAs.
传统的生物物理和化学生物学方法通过将核酸（NAs）从其天然生物环境中提取出来，来描绘结构和生物功能之间的关系。然而，累积的实验观察结果表明，NAs 的结构、动态和相互作用可能受到广泛的特定和非特定物理化学环境因素的强烈影响。这种考虑最近激发了人们对开发新型工具的兴趣，以在天然细胞环境中对 NAs 的结构进行结构特征描述。在这里，我们回顾了目前用于在天然细胞环境中对 NAs 结构进行结构特征描述的个别方法，重点介绍了新兴的细胞内 NMR 和电子顺磁共振波谱学以及细胞内单分子 FRET 领域的最新进展和发展。
