Department of Botany, Centre of Advanced Studies, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, India.
Centre for Nanobiotechnology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632 014, India.
Plant Physiol Biochem. 2018 Jun;127:403-413. doi: 10.1016/j.plaphy.2018.04.014. Epub 2018 Apr 12.
Engineered nanoparticles are utilized in agriculture for various purposes. They can be used as fertilizer, carrier for macro/micro nutrients or priming agents. Various nanoparticles are reported to have toxicity at very high doses, but at optimum concentration, they can be beneficial for plant growth and development. In the present study, low concentrations of nZVI nanoparticles were evaluated for their growth enhancement potential as seed priming agent in an aromatic rice cultivar, Oryza sativa cv. Gobindabhog. Seeds were primed with different concentrations (10, 20, 40, 80, 160 mg L) of nZVI and allowed to grow for 14 days. Seed germination and seedling growth were studied by assessing physiological, biochemical, and structural parameters at different time points. Maximum activities of hydrolytic and antioxidant enzymes, along with root dehydrogenase enzyme were observed in 20 mg L nZVI primed seeds. Priming with low doses of nZVI increased seedling vigour, as expressed by increased root and shoot length, biomass and photosynthetic pigment content. Our study also confirmed that after 14 days growth, the seedling showed absence of membrane damage, reduction in proline level and anti-oxidant enzyme activities. However, seedlings primed with 160 mg L nZVI suffered oxidative stress. SEM micrographs also revealed damage in root tissue at that concentration. AAS study confirmed uptake of nZVI by the rice plants as maximum level of iron was found in the plants treated with highest concentration (i.e. 160 mg L nZVI). Thus, nZVI at low concentrations can be considered as priming agent of rice seeds for increasing plant vigour.
纳米颗粒被应用于农业生产中，具有多种用途。它们可用作肥料、宏/微量营养素的载体或引发剂。各种纳米颗粒在高剂量下具有毒性，但在最佳浓度下，它们可以有益于植物生长和发育。在本研究中，低浓度的纳米零价铁（nZVI）被评估为作为芳香稻品种 Gobindabhog 的种子引发剂的生长增强潜力。用不同浓度（10、20、40、80、160 mg/L）的 nZVI 对种子进行引发，并允许其生长 14 天。在不同时间点评估生理、生化和结构参数来研究种子萌发和幼苗生长。在 20 mg/L nZVI 引发的种子中，观察到水解和抗氧化酶以及根脱氢酶的最大活性。低剂量的 nZVI 引发增加了幼苗活力，表现为根和茎的长度、生物量和光合色素含量的增加。我们的研究还证实，在 14 天的生长后，幼苗表现出无膜损伤、脯氨酸水平降低和抗氧化酶活性降低。然而，用 160 mg/L nZVI 引发的幼苗遭受了氧化应激。SEM 显微照片还显示在该浓度下根组织受损。AAS 研究证实了水稻植株对 nZVI 的吸收，因为在处理最高浓度（即 160 mg/L nZVI）的植物中发现了最高水平的铁。因此，低浓度的 nZVI 可以被认为是增加植物活力的水稻种子引发剂。
