Tabar A I, Sanz M L, Fernández F J, Oehling A
Departamento de Alergologia, Facultad de Medicina, Universidad de Navarra, Pamplona, Spain.
Allergol Immunopathol (Madr). 1988 Jan-Feb;16(1):11-5.
The system of IgE response undoubtedly has a special interest, given the role of this immunoglobulin on the pathogenesis of allergic diseases. In the last few years the existence of a regulator mechanism that maintains the synthesis of IgE in low but effective levels has been confirmed. This mechanism consists in the equilibrium between the actions of suppressors and enhancers. These two soluble factors present in the serum and capable of suppressing and enhancing IgE response in control rats have been identified; both have a highly selective activity on IgE, exerting their effect in an antigen-independent form and require specie-specificity. Motivated by these findings, we have applied this experimental system used in rats to humans. Healthy controls were selected as low responders of IgE, atopics as high responders, and asthmatic pollinosis considered as the prototype of allergic disease mediated by IgE. After obtaining the factors mentioned by means of a bi-directional mixed culture in a pool of lymphocytes, we proceeded to their isolation by affinity chromatography in Con-A Sepharose columns. The glycoproteins obtained were afterwards submitted to different techniques of characterization: immunoelectrophoresis in polycrilamide gel, chromatography technique, etc.
鉴于这种免疫球蛋白在过敏性疾病发病机制中的作用，IgE反应系统无疑具有特殊的研究价值。在过去几年中，已证实存在一种调节机制，可将IgE的合成维持在低但有效的水平。该机制在于抑制因子和增强因子作用之间的平衡。已经鉴定出血清中存在的这两种可溶性因子，它们能够抑制和增强对照大鼠的IgE反应；两者对IgE都具有高度选择性活性，以抗原非依赖性形式发挥作用，并且需要种属特异性。受这些发现的启发，我们将这种在大鼠中使用的实验系统应用于人类。选择健康对照作为IgE的低反应者，特应性个体作为高反应者，并将哮喘性花粉症视为由IgE介导的过敏性疾病的原型。通过在淋巴细胞池中进行双向混合培养获得上述因子后，我们通过在Con - A琼脂糖柱上进行亲和色谱法对其进行分离。随后将获得的糖蛋白进行不同的表征技术：聚丙烯酰胺凝胶免疫电泳、色谱技术等。
