Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco 186, Col. Vicentina, México, Ciudad de México 09340, Mexico.
Departamento de Preparatoria Agrícola, Universidad Autónoma Chapingo, Km. 38.5, Carretera México-Texcoco, 56230 Texcoco, Mexico.
Ultrason Sonochem. 2018 Jun;44:97-105. doi: 10.1016/j.ultsonch.2018.02.007. Epub 2018 Feb 6.
Response surface methodology was used for establishing the amplitude (72.67%) and time (17.29 min) high-intensity ultrasound (HIUS) conditions leading to an optimized faba bean protein isolate (OFPI) with lower interfacial tension, zeta potential and viscosity, and higher solubility than native faba bean protein isolate (NFPI). OFPI showed significantly higher adsorption dynamics at the air-water interface, and produced foam with significant smaller bubble diameter, higher overrun, stability and yield stress, and lower liquid drainage than NFPI. Fourier Transform Spectroscopy (FT-IR) revealed that the secondary structure of OFPI deferred from NFPI in terms of increases in β conformations (6.61% β-sheet, 19.6% β-turn, 0.8% anti-parallel β-sheet) and decreases in inter-molecular aggregates (43.54%). Multienzyme study pinpointed that the structural changes could have induced a decrease on the relative protein digestibility of OFPI respect that of NFPI. The results of this work demonstrate that HIUS technology improves the surface and foaming properties of faba bean protein isolate, which may favour the revalorisation of this crop.
响应面法用于确定高强度超声（HIUS）的幅度（72.67%）和时间（17.29 分钟）条件，以获得具有较低界面张力、较高溶解度、较低zeta 电位和粘度的优化的蚕豆分离蛋白（OFPI）。与天然蚕豆分离蛋白（NFPI）相比，OFPI在气-水界面上表现出明显更高的吸附动力学特性，产生的泡沫具有更小的气泡直径、更高的膨胀率、稳定性和屈服应力，以及更低的液体沥滤。傅里叶变换光谱（FT-IR）表明，OFPI 的二级结构与 NFPI 不同，β 构象增加（6.61% β-折叠、19.6% β-转角、0.8% 反平行 β-折叠），分子间聚集体减少（43.54%）。多酶研究表明，结构变化可能导致 OFPI 的相对蛋白质消化率相对于 NFPI 降低。这项工作的结果表明，HIUS 技术改善了蚕豆分离蛋白的表面和起泡性能，这可能有利于这种作物的再利用。
