Gao C, Zhang X, Chen J, Zhao J, Liu Y, Zhang J, Wang J
Department of Periodontology, School of Stomatology, Lanzhou University, Lanzhou, Gansu 730000, China,
Med Oral Patol Oral Cir Bucal. 2018 May 1;23(3):e344-e350. doi: 10.4317/medoral.22227.
Tongue squamous cell carcinoma is the most common squamous cell carcinoma of the head and neck. Immunotherapy has great potential in the treatment of tongue squamous cell carcinoma because of its unique advantages. However, the efficacy of immunotherapy is limited by the efficiency of antigen phagocytosis by immune cells.
We extracted dendritic cells (DCs) from human peripheral blood. Utilizing a nanosecond pulsed electric field (nsPEF), we deliver the tumour lysate protein into DCs and then incubate the DCs with PBMCs to obtain specific T cells to kill tumour cells. The biosafety of nsPEF was evaluated by the ANNEXIN V-FITC/PI kit. The efficacy of lysate protein delivery was evaluated by flow cytometry. The antitumour efficacy was tested by CCK-8 assay.
The nsPEF of the appropriate field strength can significantly improve the phagocytic ability of DCs to tumour lysing proteins and have good biosafety. The tumour cell killing rate of the nsPEF group was higher than the other group (p < 0.05).
Utilizing nsPEF to improve the phagocytic and presenting ability of DCs could greatly activate the adaptive immune cells to enhance the immunotherapeutic effect on tongue squamous cell carcinoma.
舌鳞状细胞癌是头颈部最常见的鳞状细胞癌。免疫疗法因其独特优势在舌鳞状细胞癌治疗中具有巨大潜力。然而，免疫疗法的疗效受到免疫细胞对抗原吞噬效率的限制。
我们从人外周血中提取树突状细胞（DCs）。利用纳秒脉冲电场（nsPEF），将肿瘤裂解物蛋白导入DCs，然后将DCs与外周血单个核细胞（PBMCs）共同孵育以获得特异性T细胞来杀伤肿瘤细胞。通过膜联蛋白V-异硫氰酸荧光素/碘化丙啶（ANNEXIN V-FITC/PI）试剂盒评估nsPEF的生物安全性。通过流式细胞术评估裂解物蛋白导入的效果。通过细胞计数试剂盒-8（CCK-8）检测评估抗肿瘤疗效。
适当场强的nsPEF可显著提高DCs对肿瘤裂解蛋白的吞噬能力，且具有良好的生物安全性。nsPEF组的肿瘤细胞杀伤率高于其他组（p < 0.05）。
利用nsPEF提高DCs的吞噬和呈递能力可极大地激活适应性免疫细胞，增强对舌鳞状细胞癌的免疫治疗效果。
