González-Galván M-C, Mosqueda-Taylor A, Bologna-Molina R, Setien-Olarra A, Marichalar-Mendia X, Aguirre-Urizar J-M
Departamento de Estomatologia II, Universidad del País Vasco UPV/EHU, Barrio Sarriena s/n, Leioa 48940, Bizkaia, Spain,
Med Oral Patol Oral Cir Bucal. 2018 May 1;23(3):e315-e319. doi: 10.4317/medoral.22372.
Odontogenic myxoma (OM) is a benign intraosseous neoplasm that exhibits local aggressiveness and high recurrence rates. Osteoclastogenesis is an important phenomenon in the tumor growth of maxillary neoplasms. RANK (Receptor Activator of Nuclear Factor κappa B) is the signaling receptor of RANK-L (Receptor activator of nuclear factor kappa-Β ligand) that activates the osteoclasts. OPG (osteoprotegerin) is a decoy receptor for RANK-L that inhibits pro-osteoclastogenesis. The RANK / RANKL / OPG system participates in the regulation of osteolytic activity under normal conditions, and its alteration has been associated with greater bone destruction, and also with tumor growth.
To analyze the immunohistochemical expression of OPG, RANK and RANK-L proteins in odontogenic myxomas (OMs) and their relationship with the tumor size.
Eighteen OMs, 4 small (<3 cm) and 14 large (> 3cm) and 18 dental follicles (DF) that were included as control were studied by means of standard immunohistochemical procedure with RANK, RANKL and OPG antibodies. For the evaluation, 5 fields (40x) of representative areas of OM and DF were selected where the expression of each antibody was determined. Descriptive and comparative statistical analyses were performed with the obtained data.
There are significant differences in the expression of RANK in OM samples as compared to DF (p = 0.022) and among the OMSs and OMLs (p = 0.032). Also a strong association is recognized in the expression of RANK-L and OPG in OM samples.
Activation of the RANK / RANK-L / OPG triad seems to be involved in the mechanisms of bone balance and destruction, as well as associated with tumor growth in odontogenic myxomas.
牙源性黏液瘤（OM）是一种良性骨内肿瘤，具有局部侵袭性和高复发率。破骨细胞生成是上颌肿瘤生长中的一个重要现象。RANK（核因子κB受体激活剂）是RANK-L（核因子κB配体受体激活剂）的信号受体，可激活破骨细胞。骨保护素（OPG）是RANK-L的诱饵受体，可抑制破骨细胞生成。RANK/RANKL/OPG系统在正常情况下参与溶骨活性的调节，其改变与更大程度的骨破坏以及肿瘤生长有关。
分析牙源性黏液瘤（OM）中OPG、RANK和RANK-L蛋白的免疫组化表达及其与肿瘤大小的关系。
采用RANK、RANKL和OPG抗体的标准免疫组化方法，对18例OM（4例小肿瘤（<3cm）和14例大肿瘤（>3cm））以及作为对照的18个牙囊（DF）进行研究。为进行评估，选择OM和DF代表性区域的5个视野（40倍），测定每种抗体的表达。对获得的数据进行描述性和比较性统计分析。
与DF相比，OM样本中RANK的表达存在显著差异（p = 0.022），且在小肿瘤OMS和大肿瘤OML之间也存在显著差异（p = 0.032）。此外，在OM样本中，RANK-L和OPG的表达之间存在很强的关联。
RANK/RANK-L/OPG三联体的激活似乎参与了骨平衡和破坏机制，也与牙源性黏液瘤的肿瘤生长有关。
