Aizitili Tuxunaili, Maimaiti Yushanjiang, Zhang Zhixiang, Mijiti Maihemuti
College of Agronomy, Key Laboratory of Prevention and Control of Invasive Alien Species in Agriculture & Forestry of the North-Western Desert Oasis, Ministry of Agriculture and Rural Affairs, Xinjiang Agricultural University, Urumqi, 830052, China.
MOE Key Laboratory of Bioinformatics, Center for Plant Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Virus Genes. 2025 Apr;61(2):214-219. doi: 10.1007/s11262-024-02132-0. Epub 2025 Jan 8.
A novel plant virus was identified in fig trees exhibiting ring spot symptoms through high-throughput sequencing (HTS). The complete genome sequence was successfully determined using PCR and RT-PCR techniques. The virus features a circular DNA genome of 7233 nucleotides (nt) in length, encompassing four open reading frames (ORFs). ORF1 and ORF2 encode hypothetical proteins, while ORF3 encodes a putative polyprotein with conserved domains, including a zinc finger, aspartic protease, reverse transcriptase (RT), and RNase H. ORF4 encodes a putative protein of unknown function. Comparative nucleotide sequence analysis of the RT + RNase H region reveals 84.46% and 78.82% identity with grapevine badnavirus 1 (GBV-1, MF781082.1) and fig badnavirus 1 (FBV-1, MK348055.1), respectively. Notably, this virus's genomic organization diverges from GBV-1 but is similar to FBV-1. Phylogenetic analysis demonstrates that the three isolates of this virus form a distinct clade within the badnaviruses. Based on genomic structure and phylogenetic relationships, this novel virus represents a new member of the genus Badnavirus and is proposed to be named "Fig badnavirus 2" (FBV-2).
通过高通量测序（HTS）在表现出环斑症状的无花果树上鉴定出一种新型植物病毒。利用聚合酶链反应（PCR）和逆转录聚合酶链反应（RT-PCR）技术成功测定了其完整基因组序列。该病毒具有一个长度为7233个核苷酸（nt）的环状DNA基因组，包含四个开放阅读框（ORF）。ORF1和ORF2编码假定蛋白，而ORF3编码一个具有保守结构域的假定多蛋白，包括一个锌指、天冬氨酸蛋白酶、逆转录酶（RT）和核糖核酸酶H。ORF4编码一个功能未知的假定蛋白。对RT + 核糖核酸酶H区域的比较核苷酸序列分析显示，与葡萄藤杆状DNA病毒1（GBV-1，MF781082.1）和无花果杆状DNA病毒1（FBV-1，MK348055.1）的同一性分别为84.46%和78.82%。值得注意的是，该病毒的基因组结构与GBV-1不同，但与FBV-1相似。系统发育分析表明，该病毒的三个分离株在杆状DNA病毒中形成一个独特的进化枝。基于基因组结构和系统发育关系，这种新型病毒代表杆状DNA病毒属的一个新成员，并被提议命名为“无花果杆状DNA病毒2”（FBV-2）。
