Liu Xiaofei, Ying Guangyao, Sun Chaonan, Yang Meihua, Zhang Lei, Zhang Shanshan, Xing Xiaoyan, Li Qian, Kong Weijun
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
College of Pharmacy, Jinzhou Medical University, Jinzhou, China.
Front Pharmacol. 2018 Apr 6;9:284. doi: 10.3389/fphar.2018.00284. eCollection 2018.
The high acidity and complex components of have provided major challenges for sensitive determination of trace aflatoxins. In this study, sample pretreatment of was systematically developed for sensitive high performance liquid chromatography-fluorescence detection (HPLC-FLD) after ultrasonication-assisted extraction, immunoaffinity column (IAC) clean-up and on-line post-column photochemical derivatization (PCD). Aflatoxins B, B, G, G were extracted from samples by using methanol/water (70:30, ) with the addition of NaCl. The solutions were diluted 1:8 with 0.1 M phosphate buffer (pH 8.0) to negate the issues of high acidity and matrix interferences. The established method was validated with satisfactory linearity ( > 0.999), sensitivity (limits of detection (LODs) and limits of quantitation (LOQs) of 0.15-0.65 and 0.53-2.18 μg/kg, respectively), precision (RSD <11%), stability (RSD of 0.2-3.6%), and accuracy (recovery rates of 86.0-102.3%), which all met the stipulated analytical requirements. Analysis of 28 samples indicated that one sample incubated with was positive with aflatoxin B (AFB) at 3.11 μg/kg. The strategy developed in this study also has the potential to reliably extract and sensitively detect more mycotoxins in other complex acidic matrices, such as traditional Chinese medicines, foodstuffs, etc.
[具体物质]的高酸度和复杂成分给痕量黄曲霉毒素的灵敏测定带来了重大挑战。在本研究中，系统开发了[具体物质]的样品前处理方法，用于在超声辅助萃取、免疫亲和柱（IAC）净化和在线柱后光化学衍生化（PCD）后进行灵敏的高效液相色谱 - 荧光检测（HPLC - FLD）。使用甲醇/水（70:30，[具体比例]）并添加氯化钠从样品中提取黄曲霉毒素B₁、B₂、G₁、G₂。将溶液用0.1 M磷酸盐缓冲液（pH 8.0）按1:8稀释，以消除高酸度和基质干扰问题。所建立的方法经验证具有令人满意的线性（[相关系数]＞0.999）、灵敏度（检测限（LODs）和定量限（LOQs）分别为0.15 - 0.65和0.53 - 2.18 μg/kg）、精密度（RSD＜11%）、稳定性（RSD为0.2 - 3.6%）和准确度（回收率为86.0 - 102.3%），均符合规定的分析要求。对28个[具体样品]进行分析表明，一个用[具体物质]孵育的样品中黄曲霉毒素B₁（AFB₁）呈阳性，含量为3.11 μg/kg。本研究中开发的策略也有潜力可靠地提取和灵敏检测其他复杂酸性基质中的更多霉菌毒素，如中药、食品等。
