Department of Chemistry, San Jose State University, San Jose, CA, USA.
Department of Agroindustrial Science and Technology, Federal University of Pelotas, RS, Brazil.
J Sep Sci. 2017 May;40(9):1953-1959. doi: 10.1002/jssc.201601267. Epub 2017 Apr 19.
Liquid chromatography with mass spectrometry analysis of selected food samples using silica hydride stationary phases allowed for the identification and quantification of common mycotoxins including aflatoxin B1, B2, G1, G2, ochratoxin A, and fumosinin B1. Phenyl and C columns showed relatively similar selectivity based on hydrophobicity but the phenyl phase provides an additional mechanism, π-π interaction. The most hydrophobic of the analyzed compounds was more strongly retained on the C column and also has fewer unsaturated sites, which limited the interaction with the phenyl phase. Bean, maize, rice, and wheat samples were harvested and stored under conditions conducive to fungal development, and all samples presented toxin contamination exceeding the maximum tolerable limits.
采用硅烷化固定相的液相色谱-质谱分析技术对选定的食品样本进行分析，可对常见真菌毒素进行鉴定和定量，包括黄曲霉毒素 B1、B2、G1、G2、赭曲霉毒素 A 和伏马菌素 B1。苯基和 C 柱基于疏水性表现出相对相似的选择性，但苯基相提供了额外的π-π相互作用机制。在所分析的化合物中最疏水的化合物在 C 柱上保留更强，而且不饱和位点也更少，这限制了与苯基相的相互作用。采集了豆类、玉米、大米和小麦样本，并在有利于真菌生长的条件下储存，所有样本的毒素污染都超过了最大允许限度。
