Chan Gallant K L, Hu Winnie W H, Zheng Zoey X, Huang M, Lin Yan X Y, Wang Caroline Y, Gong Amy G W, Yang X Y, Tsim Karl W K, Dong Tina T X
Shenzhen Research Institute, The Hong Kong University of Science and Technology, Shenzhen 518057, China.
Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong.
Evid Based Complement Alternat Med. 2018 Feb 28;2018:1502457. doi: 10.1155/2018/1502457. eCollection 2018.
Dementia is a persistent disorder of the mental processes and is strongly related to depression. However, the performance of current antidepression medicine is far from satisfactory. Herbal extract provides an excellent source to identify compounds for possible drug development against depression. Here, HerboChips were employed to search herbal compounds that could bind nerve growth factor (NGF). By screening over 500 types of herbal extracts, the water extract of Ginkgo Folium, the leaf of , showed a strong binding to NGF. The herbal fractions showing NGF binding were further isolated and enriched. By using LC-MS/MS analysis, one of the NGF binding fractions was enriched, which was further identified as quercetin, a major flavonoid in Ginkgo Folium. Quercetin, similar to Ginkgo Folium extract, could enhance the effect of NGF in cultured PC 12 cells, including potentiation of neurite outgrowth and phosphorylation of Erk-1/2. This is the first report of discovering an NGF binding compound by using HerboChips from herbal extracts, which could be further developed for antidepression application.
痴呆是一种持续的心理过程障碍，与抑郁症密切相关。然而，目前抗抑郁药物的疗效远不能令人满意。草药提取物为鉴定可能用于开发抗抑郁药物的化合物提供了一个极好的来源。在此，利用草药芯片来寻找能够结合神经生长因子（NGF）的草药化合物。通过筛选500多种草药提取物，银杏叶（银杏的叶子）的水提取物显示出与NGF有很强的结合力。对显示出与NGF结合的草药馏分进行进一步分离和富集。通过液相色谱-串联质谱分析，富集了其中一个与NGF结合的馏分，该馏分进一步被鉴定为槲皮素，银杏叶中的一种主要黄酮类化合物。槲皮素与银杏叶提取物类似，能够增强培养的PC12细胞中NGF的作用，包括促进神经突生长和Erk-1/2的磷酸化。这是首次报道利用草药芯片从草药提取物中发现一种与NGF结合的化合物，该化合物可进一步开发用于抗抑郁应用。
