正常个体对乙肝表面抗原疫苗接种的体液免疫和细胞免疫反应。
Humoral and cellular immune responses by normal individuals to hepatitis B surface antigen vaccination.
作者信息
Filion L G, Saginur R, Szczerbak N
机构信息
Department of Microbiology and Immunology, University of Ottawa, Ontario, Canada.
出版信息
Clin Exp Immunol. 1988 Mar;71(3):405-9.
The kinetics of the cellular and humoral responses of 30 recipients of hepatitis B vaccine were studied. All individuals exerted an HBsAg blastogenic response sometime throughout the study period but the maximum response was detected on day 28 and 56. The removal of CD8+ cells enhanced significantly the HBsAg response at the times tested, whereas treatment with anti-CD4, anti-CD8, C' and anti-CD4+ C' had no effect. Vaccination also led to the depression of phytohaemagglutinin (PHA) blastogenic response. This response was maximally suppressed 4 to 8 days after immunization at least for the primary and secondary responses and 28 days after the third dose of vaccine. The humoral response to HBsAg was detected only after the second dose of vaccine was given. The results suggest that a CD8+ cell controls the magnitude and intensity of the HBsAg blastogenic response, which may help to explain why several investigators had not been able to detect this response in hyperimmunized individuals. Primary immunization with HBsAg does lead to an expansion of B memory since a secondary response anti-HBsAg was observed.
对30名乙肝疫苗接种者的细胞和体液反应动力学进行了研究。在整个研究期间，所有个体在某个时间点都出现了乙肝表面抗原（HBsAg）增殖反应，但最大反应出现在第28天和第56天。在测试的时间点，去除CD8+细胞显著增强了HBsAg反应，而用抗CD4、抗CD8、补体（C'）和抗CD4+C'处理则没有效果。接种疫苗还导致植物血凝素（PHA）增殖反应受到抑制。至少对于初次和二次反应，这种反应在免疫后4至8天被最大程度抑制，在第三剂疫苗接种后28天被最大程度抑制。对HBsAg的体液反应仅在接种第二剂疫苗后才被检测到。结果表明，CD8+细胞控制着HBsAg增殖反应的幅度和强度，这可能有助于解释为什么一些研究者在超免疫个体中未能检测到这种反应。由于观察到了抗HBsAg的二次反应，用HBsAg进行初次免疫确实会导致B记忆细胞的扩增。
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