Department of Materials Science and Engineering, Texas A&M University, 575 Ross Street, College Station, TX, 77843, USA.
Department of Chemistry and Chemical Biology, Stevens Institute of Technology, 1 Castle Point on the Hudson, Hoboken, NJ, 07030, USA.
Adv Healthc Mater. 2018 Jun;7(11):e1800132. doi: 10.1002/adhm.201800132. Epub 2018 Apr 23.
Tailoring nanofibrous matrices-a material with much promise for wound healing applications-to simultaneously mitigate bacterial colonization and stimulate wound closure of infected wounds is highly desirable. To that end, a dual-releasing, multiscale system of biodegradable electrospun nanofibers coated with biocompatible micellar nanocarriers is reported. For wound healing, transforming growth factor-β1 is incorporated into polycaprolactone/collagen (PCL/Coll) nanofibers via electrospinning and the myofibroblastic differentiation of human dermal fibroblasts is locally stimulated. To prevent infection, biocompatible nanocarriers of polypeptide-based block copolymer micelles are deposited onto the surfaces of PCL/Coll nanofibers using tannic acid as a binding partner. Micelle-modified fibrous scaffolds are favorable for wound healing, not only supporting the attachment and spreading of fibroblasts comparable to those on noncoated nanofibers, but also significantly enhancing fibroblast migration. Micellar coatings can be loaded with gentamicin or clindamycin and exhibit antibacterial activity as measured by Petrifilm and zone of inhibition assays as well as time-dependent reduction of cellular counts of Staphylococcus aureus cultures. Moreover, delivery time of antibiotic dosage is tunable through the application of a novel modular approach. Altogether, this system holds great promise as an infection-mitigating, cell-stimulating, biodegradable skin graft for wound management and tissue engineering.
为了满足将具有很大应用前景的纳米纤维基质（一种非常适合伤口愈合应用的材料）同时用于减轻细菌定植和刺激感染伤口闭合的需求，研究人员开发了一种具有双重释放功能的、多尺度的可生物降解电纺纳米纤维涂层的生物相容性胶束纳米载体系统。在伤口愈合方面，通过电纺技术将转化生长因子-β1 掺入聚己内酯/胶原蛋白（PCL/Coll）纳米纤维中，从而局部刺激人真皮成纤维细胞的肌成纤维细胞分化。为了预防感染，使用单宁酸作为结合配体，将基于多肽的嵌段共聚物胶束的生物相容性纳米载体沉积在 PCL/Coll 纳米纤维的表面上。胶束修饰的纤维支架有利于伤口愈合，不仅支持成纤维细胞的附着和扩散，与未涂层的纳米纤维相当，而且还显著增强了成纤维细胞的迁移。胶束涂层可以装载庆大霉素或克林霉素，并表现出抗菌活性，如 Petrifilm 和抑菌圈测定以及金黄色葡萄球菌培养物的细胞计数随时间的减少所证实的。此外，通过应用新的模块化方法，可以调节抗生素剂量的释放时间。总的来说，该系统作为一种抗感染、刺激细胞、可生物降解的皮肤移植物，有望用于伤口管理和组织工程。
