Jiang Min-Hong, Hu Yue, Jiao Lin, Dong Pan, Yin Sheng, Zhao Zhi-Min, Yang De-Po, Wang Dong-Mei
a School of Pharmaceutical Sciences , Sun Yat-Sen University , Guangzhou 510006 , China.
b Guangdong Technology Research Center for Advanced Chinese Medicine , Guangzhou 510006 , China.
J Asian Nat Prod Res. 2019 Jul;21(7):627-632. doi: 10.1080/10286020.2018.1463997. Epub 2018 Apr 23.
Five fractions prepared from the crude extract of were examined in order to determine their cytotoxic potential. Under the bioassay guidance, a new labdane-type diterpenoid (), and nine known ones () along with a -labdane () were isolated from the aerial parts of . The structure elucidation was primarily based on comprehensive spectroscopic analyses, including HRESIMS, IR, and 1D and 2D NMR methods. Compound (6β-hydroxy-15,16-epoxylabda-8,13(16),14-trien-7-one) exhibited potential cytotoxicity against HeLa cell line (IC = 23.75 μM).
为了确定其细胞毒性潜力，对从[提取物名称]粗提物中制备的五个馏分进行了检测。在生物测定指导下，从[植物名称]地上部分分离出一种新的半日花烷型二萜（[化合物名称]）、九种已知化合物（[化合物名称]）以及一种β-半日花烷（[化合物名称]）。结构解析主要基于综合光谱分析，包括高分辨电喷雾电离质谱（HRESIMS）、红外光谱（IR）以及一维和二维核磁共振（NMR）方法。化合物[化合物名称]（6β-羟基-15,16-环氧半日花-8,13(16),14-三烯-7-酮）对人宫颈癌HeLa细胞系表现出潜在的细胞毒性（半数抑制浓度IC₅₀ = 23.75 μM）。
