Jiangsu Kanion Pharmaceutical Co., Ltd. Economic and Technological Development Zone, Lianyungang, 222001, People's Republic of China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, 222001, People's Republic of China.
Jiangsu Kanion Pharmaceutical Co., Ltd. Economic and Technological Development Zone, Lianyungang, 222001, People's Republic of China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang, 222001, People's Republic of China.
Phytochemistry. 2020 May;173:112223. doi: 10.1016/j.phytochem.2019.112223. Epub 2020 Feb 26.
Seven undescribed labdane diterpenoids, japonicones A-G, were isolated from the aerial parts of Leonurus japonicus Houtt. Japonicones C-G contained two keto carbonyl groups in their structures attached to C-3 and C-7, which are rare for labdane diterpenoids. The structures and absolute configurations of japonicones A-G were established by means of spectroscopic analyses (HRESIMS, 1D and 2D NMR, and ECD). Their anti-inflammatory activities were evaluated by measuring their inhibitory effects on PGE production in LPS-stimulated RAW264.7 macrophages. Japonicones A-D and G showed inhibition of PGE production with IC values in the range of 8.62-30.71 μM (the positive control paracetamol showed an IC = 5.79 μM).
从益母草地上部分分离得到了 7 个未被描述的半日花烷二萜，即 japonicones A-G。japonicones C-G 的结构中，两个酮羰基分别连在 C-3 和 C-7 位，这在半日花烷二萜中较为罕见。通过光谱分析（HRESIMS、1D 和 2D NMR、ECD）确定了 japonicones A-G 的结构和绝对构型。通过测量它们对 LPS 刺激的 RAW264.7 巨噬细胞中 PGE 产生的抑制作用，评估了它们的抗炎活性。japonicones A-D 和 G 对 PGE 产生的抑制作用具有 IC 值范围为 8.62-30.71 μM（阳性对照扑热息痛的 IC 值为 5.79 μM）。
