College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu 241000, China.
School of Science, RMIT University, Melbourne, VIC 3083, Australia.
Molecules. 2021 Feb 6;26(4):855. doi: 10.3390/molecules26040855.
Sieb. blossom is among the traditional Chinese edible flowers, and it is widely used in the food and pharmaceutical industries. Flavonoids are among the most abundant bioactive compounds in Sieb. blossom. However, the research on the extraction of flavonoids from Sieb. blossom and their immunomodulating function is insufficient. In this study, we developed a microwave-assisted enzymatic extraction of flavonoids from Sieb. blossom (FAMB) and explored their immunomodulating effect on mice with dextran sulfate sodium salt-induced colitis. The results showed that the optimum parameters for microwave-assisted enzymatic extraction of FAMB were as follows: cellulase: 2.0%; microwave power: 200 W; microwave action time: 5 min; and enzymatic hydrolysis time: 50 min. FAMB significantly promoted the lymphocyte proliferation and natural killer (NK) cell killing activity in colitis mice, and increased the concentrations of TNF-α, IFN-γ, and IL-2 in serum. FAMB also significantly reduced the apoptosis of spleen lymphocytes in these mice. These results demonstrated that the microwave-assisted enzymatic method could significantly improve the yield and efficacy extraction of FAMB. FAMB showed a good immunomodulation effect on colitis mice.
薜荔花是中国传统的食用花卉之一，在食品和制药行业中被广泛应用。黄酮类化合物是薜荔花中含量最丰富的生物活性化合物之一。然而，关于薜荔花中黄酮类化合物的提取及其免疫调节功能的研究还不够充分。本研究采用微波辅助酶法提取薜荔花中的黄酮类化合物（FAMB），并探讨其对葡聚糖硫酸钠诱导的结肠炎小鼠的免疫调节作用。结果表明，微波辅助酶法提取 FAMB 的最佳参数为：纤维素酶：2.0%；微波功率：200 W；微波作用时间：5 min；酶解时间：50 min。FAMB 显著促进了结肠炎小鼠淋巴细胞的增殖和自然杀伤（NK）细胞的杀伤活性，增加了血清中 TNF-α、IFN-γ 和 IL-2 的浓度。FAMB 还显著降低了这些小鼠脾脏淋巴细胞的凋亡。这些结果表明，微波辅助酶法可以显著提高 FAMB 的产率和提取效果。FAMB 对结肠炎小鼠具有良好的免疫调节作用。
