de Mira Fernandes Adeline, Brandão Paulo E, Dos Santos Lima Michele, de Souza Nunes Martins Maira, da Silva Thais G, da Silva Cardoso Pinto Vivian, de Paula Larissa T, Vicente Marta Elisabete S, Okuda Liria H, Pituco Edviges M
Laboratory of Bovine Viruses, Center of Research and Development of Animal Health, Biological Institute of São Paulo, São Paulo, Brazil.
Department of Preventative Veterinary Medicine and Animal Health, Faculty of Veterinary and Zootechnical Medicine, University of São Paulo, São Paulo, Brazil.
Vet Med Sci. 2018 Apr 24;4(3):183-9. doi: 10.1002/vms3.102.
Bovine coronavirus (BCoV) is one of the main aetiological agents of gastroenteritis in calves, causing significant economic damage to livestock. This study aims to characterise BCoV genetically on the basis of the N gene. A total of 114 faecal samples from beef and dairy calves with or without clinical symptoms of diarrhoea from five Brazilian states (São Paulo, Minas Gerais, Santa Catarina, Mato Grosso and Bahia) were evaluated between 2008 and 2015 by technique of Semi-nested RT-PCR for gene N and genealogical analysis. Of the 114 samples analysed, 14.91% (17/114) were positive. BCoV was detected in 22.72% (10/44) of the animals with diarrhoea and in 10% (7/70) of asymptomatic animals. BCoV was identified in calves from rural properties located in all of the regions sampled. Genealogical analysis showed that the Brazilian sequences of BCoV for the gene which codes for the N protein can be broken down into two distinct clusters, and the samples from this study were closely linked to Asian strains. These results contribute to the molecular characterization of BCoV in Brazil and are the first report of the circulation of BCoV in the states of Santa Catarina and Bahia.
牛冠状病毒（BCoV）是犊牛肠胃炎的主要病原之一，给畜牧业造成重大经济损失。本研究旨在基于N基因对BCoV进行基因特征分析。2008年至2015年间，运用半巢式RT-PCR技术对来自巴西五个州（圣保罗、米纳斯吉拉斯、圣卡塔琳娜、马托格罗索和巴伊亚）有或无腹泻临床症状的肉牛和奶牛犊牛的114份粪便样本进行了N基因检测和系统发育分析。在分析的114份样本中，14.91%（17/114）呈阳性。腹泻动物中22.72%（10/44）检测到BCoV，无症状动物中10%（7/70）检测到BCoV。在所采样的所有地区的农村养殖场的犊牛中均鉴定出BCoV。系统发育分析表明，巴西BCoV编码N蛋白的基因序列可分为两个不同的簇，本研究的样本与亚洲毒株密切相关。这些结果有助于巴西BCoV的分子特征分析，并且是圣卡塔琳娜州和巴伊亚州BCoV传播的首次报道。
