Hubei Key Laboratory of Cell Homeostasis, Laboratory of Molecular and Developmental Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P. R. China.
BGI Genomics, BGI-Shenzhen, Shenzhen 518083, P. R. China.
Gigascience. 2018 May 1;7(5). doi: 10.1093/gigascience/giy046.
The teleost fish Monopterus albus is emerging as a new model for biological studies due to its natural sex transition and small genome, in addition to its enormous economic and potential medical value. However, no genomic information for the Monopterus is currently available.
Here, we sequenced and de novo assembled the genome of M. albus and report the de novochromosome assembly by FISH walking assisted by conserved synteny (Cafs). Using Cafs, 328 scaffolds were assembled into 12 chromosomes, which covered genomic sequences of 555 Mb, accounting for 81.3% of the sequences assembled in scaffolds (∼689 Mb). A total of 18 ,660 genes were mapped on the chromosomes and showed a nonrandom distribution along chromosomes.
We report the first reference genome of the Monopterus and provide an efficient Cafs strategy for a de novo chromosome-level assembly of the Monopterus genome, which provides a valuable resource, not only for further studies in genetics, evolution, and development, particularly sex determination, but also for breed improvement of the species.
由于其天然的性别转变和小基因组，以及巨大的经济和潜在的医学价值，东亚钳蝎 Monopterus albus 正成为生物学研究的新模型。然而，目前尚无 Monopterus 的基因组信息。
在这里，我们对 M. albus 进行了测序和从头组装基因组，并报告了通过 FISH 步行辅助保守同线性（Cafs）进行的 de novochromosome 组装。使用 Cafs，将 328 个支架组装成 12 条染色体，覆盖了 555 Mb 的基因组序列，占支架中组装的序列（约 689 Mb）的 81.3%。总共将 18660 个基因映射到染色体上，并显示出沿着染色体的非随机分布。
我们报告了 Monopterus 的第一个参考基因组，并提供了一种有效的 Cafs 策略，用于 Monopterus 基因组的从头染色体水平组装，这为进一步研究遗传、进化和发育，特别是性别决定，提供了有价值的资源，也为该物种的品种改良提供了有价值的资源。
