Department of Emergency Medicine , Kyung Hee University Hospital at Kangdong , Seoul 05278 , Korea.
J Agric Food Chem. 2018 May 9;66(18):4741-4747. doi: 10.1021/acs.jafc.8b00832. Epub 2018 Apr 30.
Detection and quantitation of flavonoids are relatively difficult compared to those of other small-molecule analytes because flavonoids undergo rapid metabolic processes, resulting in their elimination from the body. Here, we report an efficient enrichment method for facilitating the analysis of vicinal-diol-containing flavonoid molecules using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. In our strategy, boronic-acid-functionalized polyacrylamide particles were used, where boronic acids bound to vicinal diols to form boronate monoesters at basic pH. This complex remained intact during the enrichment processes, and the vicinal-diol-containing flavonoids were easily separated by centrifugation and subsequent acidic treatments. The selectivity and limit of detection of our strategy were confirmed by mass spectrometry analysis, and the validity was assessed by performing the detection and quantitation of quercetin in mouse organs.
与其他小分子分析物相比，类黄酮的检测和定量较为困难，因为类黄酮会经历快速的代谢过程，从而从体内消除。在这里，我们报告了一种有效的富集方法，用于促进使用基质辅助激光解吸/电离飞行时间质谱法分析含有邻二醇的类黄酮分子。在我们的策略中，使用了硼酸官能化的聚丙烯酰胺颗粒，其中硼酸与邻二醇结合，在碱性 pH 下形成硼酸单酯。在富集过程中，该配合物保持完整，并且含有邻二醇的类黄酮通过离心和随后的酸性处理很容易分离。通过质谱分析证实了我们策略的选择性和检测限，并通过在小鼠器官中进行槲皮素的检测和定量来评估其有效性。
