Forsman Trevor T, Dueñas Maria Emilia, Lee Young Jin
Department of Chemistry, Iowa State University, Ames, Iowa, 50011, USA.
J Mass Spectrom. 2021 Mar;56(3):e4709. doi: 10.1002/jms.4709.
Derivatization reactions are commonly used in mass spectrometry to improve analyte signals, specifically by enhancing the ionization efficiency of those compounds. Vicinal diols are one group of biologically important compounds that have been commonly derivatized using boronic acid. In this study, a boronic acid with a tertiary amine was adapted for the derivatization of vicinal diol metabolites in B73 maize tissue cross-sections for mass spectrometry imaging analysis. Using this method, dozens of vicinal diol metabolites were derivatized, effectively improving the signal of those metabolites. Many of these metabolites were tentatively assigned using high-resolution accurate mass measurements. In addition, reaction interference and cross-reactivity with various other functional groups were systematically studied to verify data interpretation.
衍生化反应在质谱分析中普遍用于改善分析物信号，特别是通过提高那些化合物的电离效率。邻二醇是一类具有生物学重要性的化合物，通常使用硼酸进行衍生化。在本研究中，一种带有叔胺的硼酸被用于B73玉米组织切片中邻二醇代谢物的衍生化，用于质谱成像分析。使用该方法，数十种邻二醇代谢物被衍生化，有效改善了这些代谢物的信号。其中许多代谢物通过高分辨率精确质量测量进行了初步鉴定。此外，还系统研究了与各种其他官能团的反应干扰和交叉反应，以验证数据解读。
